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ID   PBRS2950   preliminary; circular DNA; SYN; 146 BP.
AC   K02390;
DT   01-AUG-1985 (Rel. 1, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pBRS2950 - incomplete, T7 A3 promoter/pBR322.
KW   cloning vector; drug resistance gene; mutational analysis;
KW   promoter region; tetracycline resistance.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-146
RC   pBRS206 from pBRS188 & pOP1, lambda immunity region
RC   pBRS242 from pBR322 & pBRS206
RC   pBRS240 from pBRS242
RC   pBRS246 from pBRS240
RC   pBRS2843, pBRS2852 from pBRS240
RC   pBRS2845, pBRS2846, pBRS2850 from pBRS240
RC   pBRS3077 from pBRS240
RC   pBRS2950 from pBRS240
RC   pBRS2849 from pBRS240
RC   pBRS2343 from pBRS188
RA   Savochkina L.P., Retchinsky V.O., Bibilashvili R.S.;
RT   "Stability of cloned promoter-containing fragments";
RL   Mol. Gen. Genet. 189:142-147(1983).
CC   [1] inserted strong promoters from T7 and lambda into pBR322
CC   derived promoter-probe vectors. The inserted promoters, which
CC   occurred in dissimilar environments, served as promoters for the
CC   pBR322 tetracycline resistance operon.  Promoter strength was
CC   measured by Tc resistance.  Plasmids containing T7 A2 promoters
CC   appeared to confer less Tc resistance than those containing A3
CC   promoters.
CC   NCBI gi: 209431
CC   NM (pBRS2950)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (T7)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBRS240 EcoRI
FT                   2. T7 HaeIII-HaeIII 1458bp, prom/leftmost or 4 frag
FT                   HpaI-HinfI 115bp, A3 promoter
FT                   -> pBRS2950"
FT   misc_recomb     12..13
FT                   /note="pBR322 end/T7 start"
FT   misc_recomb     127..128
FT                   /note="T7 end/pBR322 start"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7 A2 or A3 promoter"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 146 BP; 48 A; 34 C; 35 G; 29 T; 0 other;
     cgtcttcaag aattcggggc gcacactgcc tctaggtgac ttaagcgcac cacgcacgtg
     aaacaaaacg gttgacaaca tgaagtaaac acggtacgat gtaccacatg aaacgacagt
     gagtaattcg ataagcttta atgcgg