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ID   PBS44      preliminary; DNA; SYN; 10500 BP.
AC   ATCC53254;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pBS44 [BSY23] - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBS7 from linker & pRH103-delta6, P1 cre gene
RC   pBS39 from pBS7 & linker & pBM150
RC   plasmid from pBS7 & pBMTx, mouse MT gene
RC   pBS49 from pBS39 & plasmid
RC   pBS77 from pBS49
RC   pBS30 from pRH499
RC   pBS42, pBS43 from pBS30 & pJM53
RC   pBS44, pBS47 from pBS30 & linker & pCP2-4-10, yeast ILV2 gene
RA   Sauer B.;
RT   "Functional expression of the cre-lox site-specific recombination
RT   system in the yeast Saccharomyces cerevisiae";
RL   Mol. Cell. Biol. 7:2087-2096(1987).
RN   [2]
RC   pBS44 from pBR322 & LEU2 gene
RA   ;
RT   ;
RL   Unpublished (1990).
RL   U.S. Patent No. 4,959,317 dated Sept. 25, 1990.
CC   The ClaI and HindIII sites flanking the 3.8 kb ILV2 segment were
CC   modified to XhoI sites. YI-type integrating vector containing lox
CC   sites.
CC   Inserts cloned into the BamHI or SalI sites can be excised when the
CC   Cre protein is provided in trans (see also ATCC 20772, 53255).
CC   The lox sites can be isolated by an EcoRI/SalI or an XhoI/BamHI
CC   digest.
CC   The order of the major features in this plasmid is: pMB1 ori - ampR -
CC   EcoRI - lox -> - LEU2 - lox -> - XhoI - ILV2 - XhoI. [2]
CC   Note: This material is cited in a U.S and/or other Patent and may not
CC   be used to infringe the patent claims.
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: Du Pont Merck Pharmaceutical Company
CC   NM (pBS44)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)(ILV2 acetohydroxyacid synthase)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pRH43
FT                   -> pRH499
FT                   1. pRH499 HindIII
FT                   fill in
FT                   -> pBS30
FT                   1. pBS30 XhoI
FT                   2. pCP2-4-10 ClaI-HindIII 3800bp, yeast ILV2 gene
FT                   XhoI linker:XhoI linker
FT                   -> pBS44"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;