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ID   PBSF2D     preliminary; circular DNA; SYN; 15 BP.
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AC   D13770; D00301;
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DT   17-DEC-1992 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pBSF-2D - incomplete, human BSF-2/IL-6.
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KW   cloning vector; BSF-2; IL-6.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-15
RC   pT9-11 from human IL-2 & trp promoter/operator/terminator
RC   pT13SNco from pT9-11 & oligos
RC   [pBSF-2 2.5 from BSF-2 gene]
RC   pBSF-2D from pT13SNco & pBSF-2 2.5, BSF-2 gene
RC   pBSF-2F or pTBCDF-12 from pT13SNco & pBSF-2 2.5, BSF-2 gene
RA   Tonouchi N., Oouchi N., Kashima N., Kawai M., Nagase K., Okano A.,
RA   Matsui H., Yamada K., Hirano T., Kishimoto T.;
RT   "High-level expression of human BSF-2/IL-6 cDNA in Escherichia coli
RT   using a new type of expression-preparation system";
RL   J. Biochem. 104:30-34(1988).
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RN   [2]
RC   pQ from pCEIL-2 & IL-2 gene
RC   pBSF2.38 from pQ & BSF-2 gene
RC   lambda BSF2.5 from lambda gt10 & BSF-2 gene
RA   Hirano T., Yasukawa K., Hrada H., Taga T., Watanabe Y., Matsuda T.,
RA   Kashiwamura S., Nakajima K., Koyama K., Iwamatsu A., Tsunasawa S.,
RA   Sakiyama F., Matsui H., Takahara Y., Taniguchi T., Kishimoto T.;
RT   "Complementary DNA for a novel human interleukin (BSF-2) that
RT   induces B lymphocytes to produce immunoglobulin";
RL   Nature 324:73-76(1986).
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RN   [3]
RC   pT9-1 from pDR720 & pBR322 & human IL-2 gene
RC   pT9-7, pT9-7X from pBR322 & pT9-1 & linker
RC   pT9-3 from pT9-1 & trpA terminator
RC   pT9-8 from pT9-7 & trpA terminator
RC   pMI-9 from pDR720 & human IL-2 gene
RC   pTrpA from pBR322 & trpA terminator
RC   pT9-11 from pTrpA & pMI-9
RC   pT9-10 from pT9-3 & pT9-7X
RC   pT9-1t from pT9-1 & pBR322
RC   pT9-7B from pT9-1t & pBR322 & linker
RC   pT9-9 from pIN-II-A2 & pT9-7B
RC   pT9-4 from pIN-II-A2 & pT9-1
RA   Sato T., Matsui H., Shibahara S., Kobayashi T., Morinaga Y.,
RA   Kashima N., Yamasaki S., Hamuro J.;
RT   "New approaches for the high-level expression of human
RT   interleukin-2 cDNA in Escherichia coli";
RL   J. Biochem. 101:525-534(1987).
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RN   [4]
RC   pCEIL-2 from SV40 ori/early promoter
RA   Taniguchi T., Matsui H., Fujita T., Takaoka C., Kashima N.,
RA   Yoshimoto R., Hamuro J.;
RT   "Structure and expression of a cloned cDNA for human
RT   interleukin-2";
RL   Nature 302:305-310(1983).
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CC   Direct expression plasmid of BSF-2 cDNA, but high-level expression
CC   was not observed in it.
CC   NCBI gi: 220935
CC   NM (pBSF-2D)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (pT13SNco)(BSF-2)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda gt10 EcoRI
FT                   2. human BSF-2 gene
FT                   EcoRI methylase
FT                   EcoRI linker:EcoRI linker
FT                   EcoRI-EcoRI
FT                   -> lambda pBSF2.5
FT                   1. lambda pBSF2.5 EcoRI-EcoRI, human BSF-2 gene
FT                   2. pBR322 EcoRI
FT                   -> pBSF-2 2.5
FT                   1. pT13SNco BamHI-NcoI
FT                   Klenow fill in:
FT                   NcoI-MvaI linker
FT                   2. pBSF-2 2.5 EcoRI-BanI, BSF-2 gene
FT                   Klenow fill in:Klenow fill in
FT                   MvaI-
FT                   -> pBSF-2D"
FT   CDS             2..>15
FT                   /note="GEN E. coli BSF-2 gene"
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SQ   Sequence 15 BP; 3 A; 8 C; 2 G; 2 T; 0 other;
     catgccagta ccccc
//