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ID   PBSF2F     preliminary; circular DNA; SYN; 39 BP.
AC   D13771; D00302;
DT   17-DEC-1992 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pBSF-2F - incomplete, human BSF-2/IL-6.
KW   cloning vector; BSF-2; IL-2; fused protein.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-39
RC   pT9-11 from human IL-2 & trp promoter/operator/terminator
RC   pT13SNco from pT9-11 & oligos
RC   [pBSF-2 2.5 from BSF-2 gene]
RC   pBSF-2D from pT13SNco & pBSF-2 2.5, BSF-2 gene
RC   pBSF-2F or pTBCDF-12 from pT13SNco & pBSF-2 2.5, BSF-2 gene
RA   Tonouchi N., Oouchi N., Kashima N., Kawai M., Nagase K., Okano A.,
RA   Matsui H., Yamada K., Hirano T., Kishimoto T.;
RT   "High-level expression of human BSF-2/IL-6 cDNA in Escherichia coli
RT   using a new type of expression-preparation system";
RL   J. Biochem. 104:30-34(1988).
CC   pBSF-2F is a fused BSF-2 expression plasmid which was constructed
CC   with 21 amino acids of human IL-2 N-terminal region using pT13SNco,
CC   synthetic junction sequence, and BSF-2 coding sequence.
CC   High-level expression was observed in it.
CC   NCBI gi: 220936
CC   NM (pBSF-2F)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pT13SNco)(BSF-2)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. BSF-2 gene
FT                   -> pBSF-2 2.5
FT                   1. pT13SNco BamHI-BglII
FT                   Klenow fill in:
FT                   BglII-MvaI linker
FT                   2. pBSF-2 2.5 EcoRI-BanI, BSF-2 gene
FT                   Klenow fill in:Klenow fill in
FT                   MvaI-
FT                   -> pBSF-2F"
FT   CDS             <1..39
FT                   /note="GEN human IL-2-Phe-Arg-Ala-BSF-2;
FT                   NCBI gi: 220937; human IL-2 N-terminal region
FT                   from pT13SNco"
SQ   Sequence 39 BP; 8 A; 15 C; 8 G; 8 T; 0 other;
     ctgctgctag atctcttcag agccccagta cccccagga