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ID PBSF2F preliminary; circular DNA; SYN; 39 BP.
AC D13771; D00302;
DT 17-DEC-1992 (Rel. 7, Created)
DT 01-APR-1995 (Rel. 11, Last updated, Version 1)
DE E. coli plasmid vector pBSF-2F - incomplete, human BSF-2/IL-6.
KW cloning vector; BSF-2; IL-2; fused protein.
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
RC pT9-11 from human IL-2 & trp promoter/operator/terminator
RC pT13SNco from pT9-11 & oligos
RC [pBSF-2 2.5 from BSF-2 gene]
RC pBSF-2D from pT13SNco & pBSF-2 2.5, BSF-2 gene
RC pBSF-2F or pTBCDF-12 from pT13SNco & pBSF-2 2.5, BSF-2 gene
RA Tonouchi N., Oouchi N., Kashima N., Kawai M., Nagase K., Okano A.,
RA Matsui H., Yamada K., Hirano T., Kishimoto T.;
RT "High-level expression of human BSF-2/IL-6 cDNA in Escherichia coli
RT using a new type of expression-preparation system";
RL J. Biochem. 104:30-34(1988).
CC pBSF-2F is a fused BSF-2 expression plasmid which was constructed
CC with 21 amino acids of human IL-2 N-terminal region using pT13SNco,
CC synthetic junction sequence, and BSF-2 coding sequence.
CC High-level expression was observed in it.
CC NCBI gi: 220936
CC NM (pBSF-2F)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pT13SNco)(BSF-2)
CC BR ()
CC OF ()
CC OR ()
FH Key Location/Qualifiers
FT misc_feature 0..0
FT /note="1. BSF-2 gene
FT -> pBSF-2 2.5
FT 1. pT13SNco BamHI-BglII
FT Klenow fill in:
FT BglII-MvaI linker
FT 2. pBSF-2 2.5 EcoRI-BanI, BSF-2 gene
FT Klenow fill in:Klenow fill in
FT -> pBSF-2F"
FT CDS <1..39
FT /note="GEN human IL-2-Phe-Arg-Ala-BSF-2;
FT NCBI gi: 220937; human IL-2 N-terminal region
FT from pT13SNco"
SQ Sequence 39 BP; 8 A; 15 C; 8 G; 8 T; 0 other;
ctgctgctag atctcttcag agccccagta cccccagga