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ID   PBT141     preliminary; circular DNA; SYN; 6660 BP.
AC   ATCC37637;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Bacillus/Staphylococcus plasmid vector pBT141 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBT141 from pUB110 & pNM480
RC   pBT142 from pUB110 & pNM481
RC   pBT143 from pUB110 & pNM482
RC   pBT253 from pBT141 & pC194
RC   pBT251 from pBT143 & pC194
RA   Alonso J.C.;
RT   "New plasmid vectors for the construction of translational gene
RT   fusions in Bacillus subtilis";
RL   Gene 65:325-328(1988).
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--6.6; SmaI--6.6; BamHI--6.6. (ATCC staff)
CC   A 3.1 kb EcoRI/DraI fragment of pNM480 was ligated to a 3.5 kb
CC   EcoRI/PvuII fragment of pUB110.
CC   bleR = bleomycin-pheomycin resistance. (ATCC staff)
CC   One of a series (ATCC 37637 - 37639) of 3 plasmid vectors for
CC   characterization of regulatory systems in Bacillus subtilis.
CC   Inserted genes can be fused to E.coli lacZ, with one vector in the
CC   series for each of the 3 translational reading frames.
CC   Medium is 1179 V Y medium.
CC   NM (pBT141)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Bacillus subtilis 168 SB1207)(Bacillus)(Staphylococcus aureus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUB110 EcoRI-PvuII 3514bp 1036..4548..2,
FT                   \ ori/neo/bleo
FT                   2. pNM480 EcoRI-DraI 3100bp, lacZ gene
FT                   \ pUC8 231..-..1074
FT                   -> pBT141 6660bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI-SalI-PstI-HindIII-
FT                   BglII-NciI-NcoI"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT Bacillus kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT Bacillus neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
FT   CDS             0..0
FT                   /note="ANT Bacillus bleomycin resistance gene (ble)"
SQ   Sequence 39 BP; 7 A; 13 C; 11 G; 8 T; 0 other;
     gaattcccgg ggatccgtcg acctgcagcc aagcttgct