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ID   PBT7       preliminary; DNA; SYN; 2750 BP.
AC   ATCC87017;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pBT7 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBT7 from pBR322 & T7
RA   Maruyama I.N., Maruyama H.I.;
RT   "A bacteriophage T7-based expression vector, pBT7, with color
RT   selection for the recombinant";
RL   Gene 131:79-82(1993).
CC   Expression vector based on a bacteriophage T7 RNA
CC   polymerase/promoter system and allowing visual detection of inserts
CC   using lacZalpha complementation. The T7 promoter sequence has a T->A
CC   mutation at the 5' end that does not appear to affect expression. The
CC   order and orientations of the major features of the plasmid are:  <-
CC   lacZ' - HindIII - T7 promoter -> - NdeI/MCS/EcoRI - <-pMB1 ori - <-
CC   ampR. [1]
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: I.N.Maruyama
CC   NM (pBT7)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. T7, gene 10 promoter
FT                   3. E. coli, lac promoter/lacZ gene
FT                   -> pBT7 2750bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   misc_binding    0..0
FT                   /note="MCS NdeI-BamHI-SmaI-KpnI-SacI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ' gene"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
FT   promoter        0..0
FT                   /note="PRO phage T7 gene 10'"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;