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ID   PBTI7      preliminary; circular DNA; SYN; 10400 BP.
AC   ATCC37088;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli cosmid vector pBTI-7 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBTI-1 from MUA-10 & YEp13
RC   pBTI-5 from pBTI-1
RC   pBTI-6 from MUA-3 & pACYC184
RC   pBTI-7, pBTI-8 from pBTI-6
RC   pBTI-9 from pBTI-8
RC   pBTI-10 from pBTI-6 & YRp7
RA   Morris D.W., Noti J.D., Osborne F.A., Szalay A.A.;
RT   "Plasmid vectors capable of transferring large DNA fragments to
RT   yeast";
RL   DNA 1:27-36(1981).
CC   A Saccharomyces cerevisiae/E.coli cosmid shuttle vector.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pBTI-7)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (E.coli JA221)(Saccharomyces cerevisiae SHY2)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (MUA-3)(pACYC184)
CC   BR ()
CC   OF (pBTI-7)(pBTI-8)(pBTI-9 from pBTI-8)
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. MUA-3 4695bp
FT                   2. pACYC184 EcoRI-HindIII 1523bp 2..1525
FT                   -> pBTI-6 6218bp [unique EcoRI, PstI, XbaI]
FT                   1. pBTI-6 PstI 6218bp [6300bp]
FT                   2. YEp13 PstI-PstI 4043bp, yeast LEU2 gene
FT                   -> pBTI-7 10261bp [10400bp]"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_feature    0..0
FT                   /note="from pACYC184"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI-HindIII-BamHI"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_feature    0..0
FT                   /note="phage lambda cos fragment"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;