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ID   PBTRP2     preliminary; circular DNA; SYN; 5200 BP.
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AC   IG0308;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBTrp2 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   MV17 from trp promoter
RC   ptrp ED3 from pBR322 & trpE gene
RC   ptrp ED4 from pBR322 & trpE gene
RC   ptrp ED5-1 from ptrp ED3
RC   pBTrp1, pBTrp2 from pBR322 & ptrp ED5-1 & MV17, trp promoter
RC   pBR322-1H3L from pBR322 & HindIII linker
RA   Hallewell R.A., Emtage S.;
RT   "Plasmid vectors containing the tryptophan operon promoter suitable
RT   for efficient regulated expression of foreign genes";
RL   Gene 9:27-47(1980).
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CC   E. coli host must have trpR+. Tryptophan represses the trp promoter.
CC   3-beta-indolylacrylic acid induces it.
CC   NM (pBTrp2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Boehringer)
CC   HO (E.coli C600)(E.coli W3110)
CC   CP ()
CC   FN (expression of proteins harmful to E.coli)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli, trp promoter
FT                   -> MV17
FT                   1. pBR322
FT                   2. E. coli, trpE gene
FT                   -> ptrp ED3
FT                   1. ptrp ED3
FT                   -> pTRP ED5-1
FT                   1. pBR322 ClaI-PstI 3589bp 25..3614
FT                   2. pTRP ED5-1 PstI-HpaI 1500bp
FT                   3. MV17 HpaI-TaqI 33bp, trp promoter
FT                   -> pBTrp2 5200bp"
FT   promoter        0..0
FT                   /note="PRO E. coli tryptophan (trp) promoter"
FT   misc_feature    0..0
FT                   /note="HpaI-TaqI fragment of MV 17 (33 bp)"
FT   misc_binding    0..0
FT                   /note="SIT unique HpeI"
FT   misc_feature    0..0
FT                   /note="ClaI-PstI fragment from pBR322 (3600 bp)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    0..0
FT                   /note="MCS unique ClaI-NcoI-BamHI-SphI"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique NcoI"
FT   misc_feature    0..0
FT                   /note="ATG in an NcoI site"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SphI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   misc_feature    0..0
FT                   /note="PstI-HpaI fragment of pTRP ED 5-1 (1500 bp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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