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ID   PBV730TS   preliminary; DNA; SYN; 16200 BP.
AC   ATCC40455;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Streptomyces plasmid vector pBV730ts - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pMT660 from pIJ702
RC   pBV730ts from pMT660
RA   Hornemann U., Otto C.J., Hoffman G.G., Bertinuson A.C.;
RT   "Spectinomycin resistance and associated DNA amplification in
RT   Streptomyces achromogenes subsp. rubradiris";
RL   J. Bacteriol. 169:2360-2366(1987).
RN   [2]
RC   pMT660 from pIJ702
RC   pBV730ts from pMT660
RA   Hornemann U., Zhang X.Y., Otto C.J.;
RT   "Transferable Streptomyces DNA amplification and coamplification
RT   of foreign DNA sequences";
RL   J. Bacteriol. 175:1126-1133(1993).
RN   [3]
RC   pMT660 from pIJ702
RC   pBV730ts from pMT660
RA   ;
RT   ;
RL   Unpublished (1993).
RL   U.S. Patent No. 5,240,858 dated Aug. 31, 1993.
CC   pMT660 is a temperature sensitive replication mutant of pIJ702. [1]
CC   Curable vector that can be used to insert and amplify cloned sequences
CC   into host chromosomal DNA. The 10.4 kb fragment from S. achromogenes
CC   contains the complete 8.8 kb amplifiable unit with 0.8 kb direct
CC   repeats at its termini.
CC   The unique BglII site is at one end of the 1.7 kb SphI/BglII fragment
CC   that determines spectinomycin resistance.
CC   When grown at 28C in S. lividans, the plasmid is present at high copy
CC   number and permits expression of spectinomycin resistance without
CC   amplification.
CC   When grown at 39C, the plasmid is cured and streptomycin resistance is
CC   lost except when AUD-Sar 1 sequences become integrated into the host
CC   chromosome where they undergo amplification. [3]
CC   The S. achromogenes fragment contains the following restriction sites
CC   (approximate kb from one end): MluI--1.7, 2.5, 3.0, 5.4, 5.9, 9.4,
CC   10.2; SphI--2.1, 9.7; BglII--8.0. [2]
CC   Note: This material is cited in a U.S and/or other Patent and may not
CC   be used to infringe the patent claims.
CC   Deposited by: Wisconsin Alumni Research Foundation
CC   NM (pBV730ts)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Streptomyces lividans)(Streptomyces lividans)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pIJ702
FT                   mutation
FT                   -> pMT660 [temperature sensitive]
FT                   1. pMT660, spec gene/tsr gene/ori
FT                   2. S. achromogenes 10400bp
FT                   -> pBV730ts [SphI-BglII 1700bp spec]"
FT   CDS             0..0
FT                   /note="ANT Streptomyces tsrR gene"
FT   CDS             0..0
FT                   /note="ANT Streptomyces spcR gene"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   rep_origin      0..0
FT                   /note="ORI Streptomyces"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;