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ID   PC22       preliminary; circular DNA; SYN; 17500 BP.
AC   ATCC37493;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Higher plants/Agrobacterium/E.coli cosmid vector pC22 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pC10 from pBR322 & S-a
RC   pC13 from pC10 & pLJbB11 or pLJ11
RC   pC17 from pC13 & pLGV23neo
RC   pC22 from pC17 & pHSG262
RA   Simoens C., Alliotte T., Mendel R., Muller A., Schiemann J.,
RA   Van Lijsebettens M., Schell J., Van Montagu M., Inze D.;
RT   "A binary vector for transferring genomic libraries to plants";
RL   Nucleic Acids Res. 14:8073-8090(1986).
RN   [2]
RC   pRiA4 from Agrobacterium rhizogenes
RC   pRiA4b from Agrobacterium rhizogenes
RC   pRi1855 from Agrobacterium rhizogenes
RC   pRiHR1 from Agrobacterium rhizogenes
RC   pLJ series from pHSG262 & pRiHR1, oriV
RC   [pLJbB11 or pLJ11 from pHSG262 & pRiHR1, oriV]
RA   Jouanin L.;
RT   "Restriction map of an agropine-type Ri plasmid and its homologies
RT   with Ti plasmids";
RL   Plasmid 12:91-102(1984).
RN   [3]
RC   pFC13, pFC37 from pBR322 & pRiA4
RC   pAD75 from pFC13 & phage MudII1681
RC   pAD85 from pFC37 & phage MudII1734
RC   pAP113 from pAD85 & pFR10
RA   Plessis A., Robaglia C., Diolez A., Beyou A., Leach F.,
RA   Casse-Delbart F., Richaud F.;
RT   "lacZ gene fusions and insertion mutagenesis in the TL-region of
RT   Agrobacterium rhizogenes Ri plasmid";
RL   Plasmid 14:17-27(1985).
CC   A binary cosmid vector designed to introduce genomic libraries into
CC   plant cells.
CC   Medium is 1065 LB medium.
CC   NM (pC22)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli MC1061)(Agrobacterium tumefaciens)(E.coli)(higher plants)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376, ori/amp
FT                   2. S-a BamHI-BglII, oriSa
FT                   -> pC10 15900bp
FT                   1. Agrobacterium rhizogenes
FT                   -> pRiHR1 253000bp
FT                   1. lambda cos
FT                   2. kan gene
FT                   -> pHSG262 2800bp
FT                   1. pHSG262 BamHI 2800bp, lambda cos
FT                   2. pRiHR1 BamHI-BamHI 8100bp, oriV/BamHI 11 fragment
FT                   -> pLJ11 10900bp
FT                   1. pC10 remove NruI-NruI, S-a ori/8800bp
FT                   BclI linker 6bp tgatca:BclI linker 6bp tgatca
FT                   2. pLJ11 BamHI-BamHI 8100bp, Ri oriV/no cos/no kan
FT                   -> pC13 16900bp
FT                   1. Agrobacterium tumefaciens, crown gall
FT                   -> pTiC58 [Ti nopaline synthase NOS gene]
FT                   1. pTiC58, Ti nopaline synthase NOS gene
FT                   -> pLGV2381
FT                   1. pKC7 BamHI 5835bp 1848..1848, neo/kan gene
FT                   2. pLGV2381 BamHI-BamHI, NOS gene
FT                   -> pLGV23neo
FT                   1. pC13 remove BamHI-BamHI, OCS
FT                   2. pLGV23neo BclI-BamHI 1537bp, neo gene
FT                   \ pKC7 311..1848
FT                   -> pC17 17200bp
FT                   1. pC17 remove BamHI-StuI, MCS
FT                   2. pHSG262 HindIII-BamHI, lambda cos
FT                   fill in:
FT                   -> pC22 17500bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-XbaI-KpnI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI higher plants pRiHR1"
FT   promoter        0..0
FT                   /note="PRO Agrobacterium nos gene"
FT   CDS             0..0
FT                   /note="ANT E. coli CbR gene"
FT   CDS             0..0
FT                   /note="ANT E. coli spectinomycin resistance gene
FT                   (spc)"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;