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ID   PC25       preliminary; circular DNA; SYN; 1 BP.
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AC   IG5176;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pC25 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pC25 from pC10
RC   pC28 from pC10
RC   pC26 from pC25
RC   pC27 from pC25
RC   pC29 from pC28 & pC27
RA   Alliotte T., Zhu L.H., Van Montagu M., Inze D.;
RT   "Plant expression vectors with the origin of replication of the
RT   W-type plasmid Sa";
RL   Plasmid 19:251-254(1988).
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CC   MCS. oligonucleotide linker.
CC   NM (pC25)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pC10 from pBR322)(pSa)(R702)
CC   BR ()
CC   OF (pC26)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376, ori/amp
FT                   2. S-a BamHI-BglII 11500bp, oriSa
FT                   -> pC10 15900bp
FT                   1. pC10, ori/amp/oriSa
FT                   2. Ti plasmid, right T-DNA/spec gene/left T-DNA
FT                   3. R702 BamHI-BamHI 3450bp, spec/strep gene
FT                   -> pC25 16500bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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