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ID   PCAT10     preliminary; circular DNA; SYN; 3800 BP.
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AC   IG5056; M19415; M19087;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCAT10 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCAT10 from pHSG298 & pCM1 & pCM4
RC   pCAT11 from pHSG299 & pCM1 & pCM4
RC   pCAT20 from pHSG298 & pCM1 & pCM4 & pCM7
RC   pCAT21 from pHSG299 & pCM1 & pCM4 & pCM7
RC   pCAT30 from pHSG298 & pCM1 & pCM4 & pCM7
RC   pCAT31 from pHSG299 & pCM1 & pCM4 & pCM7
RC   pCAT40 from pCAT10 & pKK232-8
RA   Dobrowolski P.;
RT   "Plasmids with easily excisable cat gene cartridges";
RL   Gene 102:139-140(1991).
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CC   not original sequence. MCS.
CC   NM (pCAT10)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pHSG298)(pCM1)(pCM4)
CC   BR (pCAT11 from pHSG299 & pCM1 & pCM4)
CC   BR (pCAT20 also from pCM7)(pCAT21 also from pCM7)
CC   BR (pCAT30 also from pCM7)(pCAT31 also from pCM7)
CC   OF (pCAT40 also from pKK232-8)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSC101 9263bp, ori/tet gene
FT                   mutagenesis
FT                   -> pHSG1 9263bp [temperature sensitive, tet]
FT                   1. ColE1 6646bp
FT                   transposition
FT                   2. R1drd-19, Tn3 amp gene
FT                   -> pDMS630
FT                   1. pHSG1 EcoRI 9263bp 9262..9262
FT                   2. pDMS630 EcoRI 6646bp 6645..6645
FT                   -> pHSG124 15909bp
FT                   1. pHSG124 remove BamHI-BamHI, pSC101 ori
FT                   \ pSC101 376 to R1drd-19 Tn3 amp gene
FT                   -> pHSG401
FT                   1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   -> pSC105 16163bp
FT                   1. pSC105 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   2. pVH51 EcoRI 3847bp 3846..3846
FT                   -> pML21 10747bp
FT                   1. pHSG401
FT                   2. pML21 10747bp
FT                   -> pHSG408
FT                   1. pHSG408
FT                   -> pHSG410
FT                   1. pHSG410
FT                   -> pHSG412
FT                   1. pHSG412
FT                   2. pACYC184 4245bp
FT                   -> pHSG415
FT                   1. pHSG415
FT                   mutagenesis to temperature resistance
FT                   -> pHSG415r
FT                   1. pHSG415
FT                   -> pHSG415s
FT                   1. pHSG415s
FT                   -> pHSG415h
FT                   1. pHSG415h
FT                   -> pHSG413h
FT                   1. pHSG413h
FT                   2. pHSG262 2800bp
FT                   -> pHSG439
FT                   1. pHSG415r HaeII-HaeII, ori
FT                   2. pHSG439 HhaI-HaeII, Tn9 cat gene
FT                   \ pACYC184 cat gene
FT                   \ pBR328 HhaI-HhaI 1364bp 3170..4534, cat gene
FT                   3. pUC8 HaeII-HaeII 424bp 5..429, MCS/lacZ'
FT                   -> pHSG575 3610bp [cat gene]
FT                   1. pHSG575 remove TaqI-HindIII, MCS
FT                   fill in:fill in
FT                   -> pHSG579 [cat gene]
FT                   1. pNO1523, lambda cos
FT                   -> pHSG591
FT                   1. pHSG579 remove PstI-EcoRI, MCS
FT                   nuclease:
FT                   2. pHSG591 HaeIII-EcoRI, MCS
FT                   -> pHSG587
FT                   1. pUC19 remove HindIII-HaeII 237bp 448..685,
FT                   \ lacZ gene 5' end 2449bp
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. pNO1523, strep gene
FT                   -> pHSG1311
FT                   1. pHSG1311 AhaII-EcoRI 2221bp 397..2618,strep gene to
FT                   \ lacZ gene 3' end
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pHSG367 2221bp [EcoRI, HindIII, no PvuII]
FT                   1. pHSG587 HaeII-HindIII
FT                   nuclease:
FT                   2. pHSG367 PstI-HindIII
FT                   nuclease:
FT                   -> pHSG1327
FT                   1. pHSG1327 remove HgaI-BamHI, amp/MCS
FT                   fill in:
FT                   -> pHSG1330
FT                   1. pHSG415s
FT                   -> pHSG418
FT                   1. pHSG418
FT                   -> pHSG425
FT                   1. pHSG415
FT                   2. pHC79, lambda cos
FT                   -> pHSG422
FT                   1. pHSG425
FT                   2. pHSG422
FT                   -> pHSG429
FT                   1. pHSG1330 SalI-HindIII, ori
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. pHSG429 HaeII-HaeII 1430bp, kan gene/#V00359
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pHSG1339 2231bp
FT                   1. pHSG1339 TaqI 2231bp 2055..2055
FT                   T4 DNA polymerase
FT                   2. pUC18 HaeII-HaeII 445bp 240..685, MCS/lacZ' gene
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pHSG298 2676bp
FT                   1. pHSG298 remove SalI-BamHI 12bp, MCS/pUC18 418..430
FT                   \ 2664bp
FT                   2. pCM4 BamHI-EcoRI 522bp 306..828, cat gene
FT                   \ BamHI = 306 1086
FT                   3. pCM1 EcoRI-SalI 532bp 828..1360, cat gene
FT                   \ SalI = 581 1360
FT                   -> pCAT10 3718bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="MCS from pHSG298"
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SQ   Sequence 51 BP; 10 A; 15 C; 16 G; 10 T; 0 other;
     gaattcgagc tcggtacccg gggatccgtc gacctgcagg catgcaagct t
//