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ID   PCAT19     preliminary; circular DNA; SYN; 1053 BP.
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AC   IG5016;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pCAT19 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCAT19 from pBR325 & pUC19
RA   Fuqua W.C.;
RT   "An improved chloramphenicol resistance gene cassette for
RT   site-directed marker replacement mutagenesis";
RL   Biotechniques 12:223-225(1992).
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CC   MCS, not original sequence.
CC   NM (pCAT19)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR325)(pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR325 BstBI-BstBI 952bp, Tn9 cat gene
FT                   Klenow, fill in
FT                   2. pUC19 HincII
FT                   -> plasmid [EcoRI then cat gene]
FT                   1. plasmid small SacI-HindIII, cat gene
FT                   mung bean nuclease, fill in
FT                   2. pUC19 HincII
FT                   -> pCAT19 [pUC19 MCS at both ends]"
FT   misc_binding    1..53
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-
FT                   HincII/HindIII-SphI-PstI-HindIII/BstBI"
FT   misc_feature    0..0
FT                   /note="pBR325 BstBI-BstBI 952bp, containing Tn9 CAT
FT                   gene, blunt-ended, inserted into pUC19 HincII"
FT   CDS             54..1007
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml),
FT                   from Tn9"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    1008..1053
FT                   /note="MCS BstBI/HindIII-XbaI-BamHI-SmaI-KpnI-
FT                   SacI/HincII-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1053 BP; 19 A; 28 C; 31 G; 21 T; 954 other;
     gtgaattcga gctcggtacc cggggatcct ctagagttgc atgcctgcag gtcnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac tctagaggat
     ccccgggtac cggacctgca ggcatgcaag ctt
//