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ID   PCATF35    preliminary; circular DNA; SYN; 1 BP.
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AC   IG7008;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCATF35 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   plambdaTF15 from lambda & Xenopus TFIIIA promoter
RC   pUC13CAT from pUC13 & cat gene
RC   pCATF9, pCATF15, pCATF16, pCATF30 from pUC13CAT & plambdaTF15
RC   pCATF35 from pCATF9 & pCATF16
RC   pCATF34 from pCATF9 & pCATF15
RC   pCATF36 from pCATF9 & pCATF30
RA   Hall R.K., Taylor W.L.;
RT   "Transcription factor IIIA gene expression in Xenopus oocytes
RT   utilizes a transcription factor similar to the major late
RT   transcription factor";
RL   Mol. Cell. Biol. 9:5003-5011(1989).
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RN   [2]
RC   NPYlambda37 from Charon 4A & human placental DNA
RC   pNPYlambda37-6 from pUC13 & NPYlambda37
RC   pNPYlambdaPstA12, pNPYlambdaPstG74 from pUC13 & NPYlambda37
RC   pUC13cat3 from pUC13 & pCatB', cat gene
RC   pCatNPYF6, pCatNPYA1 from pUC13cat3 & NPY promoter
RA   Minth C.D., Andrews P.C., Dixon J.E.;
RT   "Characterization, sequence, and expression of the cloned human
RT   neuropeptide-Y gene";
RL   J. Biol. Chem. 261:11974-11979(1986).
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RN   [3]
RC   pNPY3-75 from human pheochromocytoma
RA   Minth C.D., Bloom S.R., Polak J.M., Dixon J.E.;
RT   "Cloning, characterization, and DNA sequence of a human cDNA
RT   encoding neuropeptide tyrosine";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:4577-4581(1984).
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CC   NM (pCATF35)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E. coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR (pCAT)(pCAT10)(pCAT19)
CC   OF (pOEV)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   2. Xenopus BglII-PstI 568bp, TFIIIA prom -425 to 143
FT                   -> plambdaTF15
FT                   1. pUC13 SalI 2680bp 249..249
FT                   2. pSV2-cat RsaI-RsaI 1571bp 2106..3677,
FT                   \ SV40 promoter/cat gene
FT                   -> pUC13CAT 4300bp
FT                   1. pUC13CAT SmaI 4300bp, pUC13 268..268
FT                   2. plambdaTF15, BglII-PstI 568bp,
FT                   \ Xenopus TFIIIA promoter -425 to 143
FT                   BAL31 nuclease
FT                   PstI-PstI 432bp, 3' TFIIIA promoter
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pCATF9 4700bp [-425 to 7]
FT                   1. pUC13CAT SmaI 4300bp, pUC13 268..268
FT                   2. plambdaTF15, BglII-PstI 568bp,
FT                   \ Xenopus TFIIIA promoter -425 to 143
FT                   BAL31 nuclease
FT                   BglII-BglII 468bp, 5' TFIIIA promoter
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pCATF16 4700bp [-325 to 143]
FT                   1. pCATF9 remove small SacI-NsiI, pUC13 SacI 277
FT                   2. pCATF16 SacI-NsiI
FT                   -> pCATF35 4700bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//