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ID   PCB101     preliminary; circular DNA; SYN; 11300 BP.
AC   ATCC39020;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Bacillus/Brevibacterium/Corynebacterium plasmid vector pCB101 - incom.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCB101 from pCG11 & pUB110
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,710,471 dated Dec. 1, 1987.
CC   The original vials from the depositor are being used for distribution.
CC   (ATCC staff)
CC   This was constructed by ligating pCG11 (ATCC 39022) linearized with
CC   BglII to pUB110 linearized with BamHI.
CC   Cloning into the BglII site inactivates kanR.
CC   This construct contains the following restriction sites (in
CC   approximate kb from the unique PstI site):EcoRI--1.1, 7.1;
CC   HincII--3.2, 5.5, 8.8, 9.3; BglII--9.1.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pCB101)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Corynebacterium glutamicum LA103)
CC   HO (Bacillus subtilis)(Brevibacterium)(Corynebacterium glutamicum)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. Corynebacterium
FT                   -> pCG1
FT                   1. pCG1, ori/kan gene
FT                   -> pCG11
FT                   1. pCG11 BglII, kan gene
FT                   2. pUB110 BamHI, complete
FT                   -> pCB101"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII"
FT   rep_origin      0..0
FT                   /note="ORI Corynebacterium pCG1"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli spectinomycin resistance gene
FT                   (spc)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;