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ID   PCB2       preliminary; circular DNA; SYN; 1 BP.
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AC   ATCC37270;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pCB2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCB1, pCB2 from pBR322 & B.amyloliquefaciens DNA
RC   pCB3 from pBR322 & pCB2
RC   pCB4, pCB5, pCB6 from pCB3 & pBR322
RA   Childs J.D., Birnboim H.C.;
RT   "Plasmids containing multiple copies of cloned DNA for use as DNA
RT   size markers for gel electrophoresis, constructed by
RT   bacteriophage T4 transduction";
RL   Plasmid 11:82-91(1984).
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CC   Use with other pCB plasmids (ATCC 37269-37274) as gel electrophoresis
CC   size markers for both covalently closed circular and linear DNA:
CC   5842/11684 bp.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCB2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII
FT                   2. pCB1 HindIII-HindIII 740bp
FT                   -> pCB2 5840bp [1480bp insert]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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