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ID   PCE10      preliminary; circular DNA; SYN; 5300 BP.
AC   ATCC37235;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Staphylococcus plasmid vector pCE10 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pMK148 from S. simulans
RC   pCT20, pCT4, pCT3 from pC194 & pMK148
RC   pE12 from pI258, Tn551 ery
RC   pTE15, pCE10 from pCT20 & pE12
RA   Keller G., Schleifer K.H., Gotz F.;
RT   "Construction and characterization of plasmid vectors for cloning
RT   in Staphylococcus aureus and Staphylococcus carnosus";
RL   Plasmid 10:270-278(1983).
CC   Constructed by inserting the 2.15 and 0.1 kb HindIII fragments from
CC   pE12 into the HindIII site of pC194.
CC   The BclI site interrupts the erythromycin resistance gene. [1]
CC   Medium is 3 nutrient agar.
CC   NM (pCE10)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Staphylococcus aureus SA113)
CC   HO (Staphylococcus aureus)(Staphylococcus carnosus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. S. simulans, tet gene
FT                   -> pMK148 4500bp
FT                   1. pC194 HindIII 2910bp 2436..2436
FT                   2. pMK148 HindIII-HindIII 2400bp, tet gene/HindIII A
FT                   -> pCT20 5300bp
FT                   1. pI258 HhaI-HhaI 2500bp, Tn551 ery
FT                   -> pE12 2500bp
FT                   1. pCT20 remove HindIII-HindIII 2400bp, tet gene
FT                   2. pE12 HindIII-HindIII 2400bp, ery/HindIII A+C frags
FT                   -> pCE10 5300bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII-HaeIII-SstI-BclI"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus pC194"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus pE12"
FT   CDS             0..0
FT                   /note="ANT Staphylococcus chloramphenicol
FT                   acetyltransferase gene (cat); chloramphenicol
FT                   resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT Staphylococcus erythromycin resistance gene
FT                   (ery)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;