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ID   PCE10      preliminary; circular DNA; SYN; 5300 BP.
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AC   ATCC37235;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Staphylococcus plasmid vector pCE10 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pMK148 from S. simulans
RC   pCT20, pCT4, pCT3 from pC194 & pMK148
RC   pE12 from pI258, Tn551 ery
RC   pTE15, pCE10 from pCT20 & pE12
RA   Keller G., Schleifer K.H., Gotz F.;
RT   "Construction and characterization of plasmid vectors for cloning
RT   in Staphylococcus aureus and Staphylococcus carnosus";
RL   Plasmid 10:270-278(1983).
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CC   Constructed by inserting the 2.15 and 0.1 kb HindIII fragments from
CC   pE12 into the HindIII site of pC194.
CC   The BclI site interrupts the erythromycin resistance gene. [1]
CC   Medium is 3 nutrient agar.
CC   NM (pCE10)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Staphylococcus aureus SA113)
CC   HO (Staphylococcus aureus)(Staphylococcus carnosus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. S. simulans, tet gene
FT                   -> pMK148 4500bp
FT                   1. pC194 HindIII 2910bp 2436..2436
FT                   2. pMK148 HindIII-HindIII 2400bp, tet gene/HindIII A
FT                   -> pCT20 5300bp
FT                   1. pI258 HhaI-HhaI 2500bp, Tn551 ery
FT                   -> pE12 2500bp
FT                   1. pCT20 remove HindIII-HindIII 2400bp, tet gene
FT                   2. pE12 HindIII-HindIII 2400bp, ery/HindIII A+C frags
FT                   -> pCE10 5300bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII-HaeIII-SstI-BclI"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus pC194"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus pE12"
FT   CDS             0..0
FT                   /note="ANT Staphylococcus chloramphenicol
FT                   acetyltransferase gene (cat); chloramphenicol
FT                   resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT Staphylococcus erythromycin resistance gene
FT                   (ery)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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