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ID   PCE30      preliminary; circular DNA; SYN; 4001 BP.
AC   M36426; ATCC37830;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pCE30 - incomplete, promoter.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCE30 from pUC9 & pMY17-3, lambda cI857 gene/pR/pL
RC   pCE33 from pCE30 & pMOB45
RC   pND201 from pCE30 & linker
RC   pPT150 from pCE30 & pBR322
RC   pMA200U from pCE30 & pTZ18U
RA   Elvin C.M., Thompson P.R., Argall M.E., Hendry P., Stamford N.P.,
RA   Lilley P.E., Dixon N.E.;
RT   "Modified bacteriophage lambda promoter vectors for overproduction
RT   of proteins in Escherichia coli";
RL   Gene 87:123-126(1990).
CC   Constructed by inserting a 1349 bp fragment from pMY17-3 containing
CC   the cI857 gene and tandem lambda PR and PL promoters into pUC9. The
CC   promoters are immediately upstream of the cloning sites.
CC   The sequence surrounding the cloning sites has been used to design
CC   primers for direct sequencing, including the M13 universal primer.
CC   Because cI857 is expressed by the plasmid itself, from its natural
CC   promoter PM, the vector may be used in virtually any E.coli strain.
CC   Expression vector containing primer sites useful for sequencing and
CC   encoding cI857. (ATCC staff)
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.0; BamHI/EcoRI--4.0; BglI--2.9, 1.1. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 208976
CC   NM (pCE30)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli AN1459)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. lambda dv021, O gene N-terminal region
FT                   -> pOA-4
FT                   1. pBR322
FT                   2. lambda dv021
FT                   -> pBlambda4
FT                   1. pBR322
FT                   2. lambda, cI857ts gene/pL promoter/pR promoter
FT                   -> pMY12-6
FT                   1. pMY12-6
FT                   2. pOA-4
FT                   3. pBlambda4
FT                   -> pMY17-3
FT                   1. pUC9 PstI 2665bp 246..246
FT                   2. pMY17-3 PstI-PstI 1349bp, lambda cI857 gene/pR/pL
FT                   -> pCE30 4001bp"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI-SmaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PL"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PR"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 143 BP; 37 A; 35 C; 40 G; 31 T; 0 other;
     agggcagcat tcaaagcaga aggctttggg gtgtgtgata cgaaacgaag cattgggatc
     cccgggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     caacttaatc gccttgcagc aca