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ID   PCFM414    preliminary; circular DNA; SYN; 4465 BP.
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AC   ATCC40076;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pCFM414 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCFM414 from pSC101 & copA/copB genes
RC   pCFM526 from NR1 & pBEU1
RC   pCFM536 from lambda, pL promoter/Toop terminator
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,703,008 dated Oct. 27, 1987.
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RN   [2]
RC   pCFM414 from pSC101 & copA/copB genes
RC   pCFM526 from NR1 & pBEU1
RC   pCFM536 from lambda, pL promoter/Toop terminator
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,710,473 dated Dec. 1, 1987.
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RN   [3]
RC   pCFM4722 from copA/copB genes & kan gene & lambda, pL promoter
RA   Morris C.F.;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,710,474 dated Dec. 1, 1987.
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CC   There is no XbaI site even though restriction site cluster indicates
CC   one. [2]
CC   This is a plasmid vector that maintains 20 copies/cell at 30C but
CC   replicates in an uncontrolled manner at 37C until cell death occurs
CC   (doubling copy number every 15 min.). [2]
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--4.3. (ATCC staff)
CC   The vector was contructed from the following sequences (EcoRI site as
CC   nucleotide 1):0-35, multiple cloning site; 37-235, terminator;
CC   416-1259, copA, copB; 1260-2879, repA, ori; 2880-4008, bla; 4089-4465,
CC   stability sequence. [2]
CC   The sequence from nucleotides 4089 to 4465 were derived from pSC101
CC   (ATCC 3702) and can be removed from this construct by an EcoRI/SstI
CC   double digest. [2]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCFM414)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli C600)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSC101, ori/amp gene
FT                   2. copA/copB genes
FT                   -> pCFM414"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-HpaI-XbaI-NcoI-HindIII-XhoI-BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-EcoRI-HindIII-HpaI-NcoI-
FT                   SstI-XhoI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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