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ID   PCFM526    preliminary; circular DNA; SYN; 4650 BP.
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AC   ATCC39932;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pCFM526 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCFM414 from pSC101 & copA/copB genes
RC   pCFM526 from NR1 & pBEU1
RC   pCFM536 from lambda, pL promoter/Toop terminator
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,703,008 dated Oct. 27, 1987.
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RN   [2]
RC   pCFM414 from pSC101 & copA/copB genes
RC   pCFM526 from NR1 & pBEU1
RC   pCFM536 from lambda, pL promoter/Toop terminator
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,710,473 dated Dec. 1, 1987.
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CC   This vector contains the copA region from NR1, the copB region from
CC   Beu1, & the repA and ori sequences of NR1. There is a G/C to A/T
CC   mutation at bp 598 in the copB coding region. [2]
CC   Temperature sensitive. Grow at 28C.
CC   Ampicillin is the selection marker for pCFM526, kanamycin for pMW1.
CC   This is an expression plasmid containing a lambdaPL promoter, a
CC   ribosome binding site, a multiple cloning sequence, and a lambda Toop
CC   terminator. [2]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCFM526)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli AM7/pMW1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. NR1, ori/copA
FT                   2. pBEU1, copB
FT                   -> pCFM526 [lambda pL]"
FT   misc_binding    0..0
FT                   /note="MCS HgiA-ClaI-XbaI-EcoRI-HpaI-KpnI-NcoI-
FT                   HindIII-XhoI-BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI-XhoI-BamHI-HindIII-HpaI-
FT                   KpnI-NcoI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli R1"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PL"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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