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ID   PCG1       preliminary; circular DNA; SYN; 3000 BP.
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AC   ATCC31808;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Brevibacterium/Corynebacterium plasmid vector pCG1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCG1 from Corynebacterium glutamicum strain 225-57
RC   pCG2 from pCG1
RC   pCG4 from pCG2
RC   pCG11 from pCG1 & pCG4
RA   ;
RT   ;
RL   Unpublished (1985).
RL   U.S. Patent No. 4,500,640 dated Feb. 19, 1985.
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RN   [2]
RC   pCG1 from Corynebacterium glutamicum strain 225-57
RC   pCG2 from pCG1
RC   pCG4 from pCG2
RC   pCG11 from pCG1 & pCG4
RA   ;
RT   ;
RL   Unpublished (1986).
RL   U.S. Patent No. 4,617,267 dated Oct. 14, 1986.
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CC   A general purpose shuttle vector.  Natural isolate from strain 225-57.
CC   [1]
CC   Contains no BamHI, PstI, SalI, or KpnI sites. [2]
CC   Restriction digests of the plasmid give the following sizes (kb):
CC   EcoRI--2.95; HindIII--2.5, 0.4; HincII--2.2, 0.8. [2]
CC   Medium is 1445 3 plus streptomycin (20 mcg/ml).
CC   NM (pCG1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Corynebacterium glutamicum 225-57)
CC   HO (Brevibacterium)(Corynebacterium)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Corynebacterium glutamicum strain 225-57
FT                   -> pCG1"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI Corynebacterium pCG1"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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