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ID   PCG11      preliminary; circular DNA; SYN; 6700 BP.
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AC   ATCC39022;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Brevibacterium/Corynebacterium plasmid vector pCG11 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCG1 from Corynebacterium glutamicum strain 225-57
RC   pCG2 from pCG1
RC   pCG4 from pCG2
RC   pCG11 from pCG1 & pCG4
RA   ;
RT   ;
RL   Unpublished (1985).
RL   U.S. Patent No. 4,500,640 dated Feb. 19, 1985.
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RN   [2]
RC   pCG1 from Corynebacterium glutamicum strain 225-57
RC   pCG2 from pCG1
RC   pCG4 from pCG2
RC   pCG11 from pCG1 & pCG4
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,710,471 dated Dec. 1, 1987.
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CC   The original vials from the depositor are being used for distribution.
CC   (ATCC staff)
CC   This is a general purpose cloning vector for Corynebacterium hosts.[2]
CC   pCG11 was constructed by cloning a 3.7 kb BamHI fragment of pCG4 (ATCC
CC   31830) into the BglII site of pCG1 (ATCC 31808). [1]
CC   Contains the following sites separated by (kb) (approx): PstI - 1.07
CC   - EcoRI - 1.95 - HincII - 0.95 - EcoRI - 1.29 - HincII - 0.86 -
CC   BglII - 0.51 - PstI. [1]
CC   Medium is 1445 3 plus streptomycin (20 mcg/ml).
CC   NM (pCG11)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Corynebacterium glutamicum LA103)
CC   HO (Brevibacterium)(Corynebacterium)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pCG1
FT                   -> pCG2
FT                   1. pCG2
FT                   -> pCG4
FT                   1. pCG1
FT                   2. pCG4
FT                   -> pCG11 6700bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI-BglII"
FT   rep_origin      0..0
FT                   /note="ORI Corynebacterium pCG4"
FT   CDS             0..0
FT                   /note="ANT Corynebacterium spectinomycin resistance
FT                   gene (spc)"
FT   CDS             0..0
FT                   /note="ANT Corynebacterium streptomycin resistance
FT                   gene (str)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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