back Return to this vector's summary.
ID   PCG150     preliminary; circular DNA; SYN; 168 BP.
XX
AC   M24051; M21655; M24052;
XX
DT   15-JUN-1989 (Rel. 4, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   E. coli K12 plasmid vector pCG150 - incomplete, maltose binding.
XX
KW   cloning vector; maltose binding fusion protein.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-60, first
RP   1-48, second
RC   pPL-5A from malE gene
RC   pCG806 from pUC18 & pmalE, malE gene
RC   pCG150 from pUC18 & pPL-5A, malE gene
RC   pCG325 from pCG150 & pMLB1034
RC   pPL-6A' from pHI28 & pmalE & pCH2
RC   pCG228 from pUC18 & pGW4400
RC   pCG410 from pCG228 & pCG150
RC   pCG828 from pCG228 & pCG806
RC   pCG1001 from pACYC184 & modification methylase
RA   Guan C.D., Li P., Riggs P.D., Inouye H.;
RT   "Vectors that facilitate the expression and purification of foreign
RT   peptides in Escherichia coli by fusion to maltose-binding protein";
RL   Gene 67:21-30(1988).
XX
RN   [2]
RC   pmalE from malE gene
RA   Lee C.;
RT   ;
RL   Unpublished (1988).
XX
RN   [3]
RC   pHI28 from phoA gene
RA   Inouye H.;
RT   ;
RL   Unpublished (1988).
XX
RN   [4]
RC   pCH58 from pKT218 & pHI1, phoA gene
RC   pCH39 from pKT279 & pHI1, phoA gene
RC   pCH8, pCH9, pCH40 from pKT280 & pHI1, phoA gene
RC   pCH2, pCH4 from pKT287 & pHI1, phoA gene
RC   pCH38 from pBR322 & pCH2
RC   pCH45 from pCH2 & lamB gene
RC   pCH57 from pCH2 & ompF gene
RA   Hoffman C.S., Wright A.;
RT   "Fusions of secreted proteins to alkaline phosphatase: an approach
RT   for studying protein secretion";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:5107-5111(1985).
XX
RN   [5]
RC   pPst201 from pBR322 & PstI endonuclease/modification methylase
RC   pMe101, pMe201, pMe301, pMe401 from pPst201
RC   pXba3, pXba6 from pBR322 & PstI endonuclease/modification methylase
RA   Walder R.Y., Walder J.A., Donelson J.E.;
RT   "The organization and complete nucleotide sequence of the PstI
RT   restriction-modification system";
RL   J. Biol. Chem. 259:8015-8026(1984).
XX
RN   [6]
RC   pHC2 or pSM5 from lamB gene
RA   Clement J.M., Hofnung M.;
RT   "Gene sequence of the lambda receptor, an outer membrane protein
RT   of E. coli K12";
RL   Cell 27:507-514(1981).
XX
RN   [7]
RC   pHC2 or pSM5 from lamB gene
RA   Michaelis S.;
RT   ;
RL   Unpublished (1985).
XX
RN   [8]
RC   pHI1 from phoA gene
RA   Inouye H., Michaelis S., Wright A., Beckwith J.;
RT   "Cloning and restriction mapping of the alkaline phosphatase
RT   structural gene (phoA) of Escherichia coli and generation of
RT   deletion mutants in vitro";
RL   J. Bacteriol. 146:668-675(1981).
XX
RN   [9]
RC   pGW4400 from pBR322 & PstI endonuclease/modification methylase
RA   Wilson G.;
RT   ;
RL   Unpublished (1984).
XX
CC   NCBI gi: 208292 & 208293
CC   NM (pCG150)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli K-12)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. malE gene
FT                   -> pPL-5A
FT                   1. pUC18 remove BamHI-KpnI, MCS
FT                   2. pPL-5A EcoRI-HinfI 1100bp, malE gene
FT                   fill in:fill in
FT                   -> pCG150"
FT   misc_recomb     54..55
FT                   /note="E. coli lac promoter end/E. coli malE start;
FT                   first"
FT   CDS             <1..>60
FT                   /note="GEN E. coli K12 fusion protein;
FT                   NCBI gi: 208295; first"
FT   misc_recomb     6..7
FT                   /note="E. coli malE end/E. coli lacZ start; second"
FT   CDS             <1..>48
FT                   /note="GEN E. coli K12 fusion protein;
FT                   NCBI gi: 208296; second"
XX
SQ   Sequence 168 BP; 26 A; 29 C; 32 G; 21 T; 60 other;
     atgaccatga ttacgaattc gagctcggta cccgggcgag ctcggtaccc attcgaagaa
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     gcgcagactg gggatcctct agagtcgacc tgcaggcatg caagcttg
//