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ID   PCG4       preliminary; circular DNA; SYN; 28000 BP.
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AC   ATCC53882; ATCC53880; ATCC31871; ATCC31870; ATCC31869; ATCC31830;
AC   ATCC31835; ATCC31837; ATCC31838; ATCC31839;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Brevibacterium/Corynebacterium plasmid vector pCG4 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCG1 from Corynebacterium glutamicum strain 225-57
RC   pCG2 from pCG1
RC   pCG4 from pCG2
RC   pCG11 from pCG1 & pCG4
RC   plasmid from Corynebacterium glutamicum 225-250, ATCC31830
RC   plasmid from Corynebacterium glutamicum L 15, ATCC31835
RC   plasmid from Corynebacterium herculis, ATCC31837
RC   plasmid from Brevibacterium flavum, ATCC31838
RC   plasmid from Corynebacterium glutamicum, ATCC31839
RA   ;
RT   ;
RL   Unpublished (1985).
RL   U.S. Patent No. 4,500,640 dated Feb. 19, 1985.
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RN   [2]
RC   pCG1 from Corynebacterium glutamicum strain 225-57
RC   pCG2 from pCG1
RC   pCG4 from pCG2
RC   pCG11 from pCG1 & pCG4
RC   plasmid from Corynebacterium glutamicum L 15, ATCC31835
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,681,847 dated July 21, 1987.
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CC   FERM-BP 529, FERM-BP 530, FERM P-5939, FERM-P 5941, FERM-P 5942,
CC   FERM-P 5943, FERM-P 5952, FERM-P 5953 [1]
CC   A general purpose cloning vector for use in Corynebacterium or
CC   Brevibacterium. [1] [2]
CC   Contains sites for EcoRI (4), BamHI (7), HindIII (9), PstI (6) &
CC   SalI (6).
CC   EcoRI digest gives bands of 8.72, 8.03, 7.06, 6.27 kb.  PstI digest
CC   gives 11.75, 7.06, 5.38, 2.23, 1.87, 1.17 kb. [1]
CC   The depositor recommends a medium of 2% powdered bouillon and 0.5%
CC   yeast extract. (personal communication)
CC   Medium is nutrient agar (ATCC medium number 3) 30C
CC   Deposited by: Kyowa Hakko Kogyo Co., Ltd.
CC   NM (pCG4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)(Kyowa Hakko Kogyo Co)
CC   HO (Corynebacterium glutamicum L 15)(Corynebacterium herculis)
CC   HO (Brevibacterium)(Corynebacterium)(Corynebacterium glutamicum)
CC   HO (Corynebacterium glutamicum 225-250)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE ()
CC   PA (Corynebacterium ATCC 31830)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pCG1
FT                   -> pCG2
FT                   1. pCG2
FT                   -> pCG4 28000bp"
FT   rep_origin      0..0
FT                   /note="ORI Corynebacterium pCG4"
FT   CDS             0..0
FT                   /note="ANT Corynebacterium spectinomycin resistance
FT                   gene (spc)"
FT   CDS             0..0
FT                   /note="ANT Corynebacterium streptomycin resistance
FT                   gene (str)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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