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ID   PCG806     preliminary; circular DNA; SYN; 63 BP.
AC   M21656;
DT   15-JUN-1989 (Rel. 4, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli K12 plasmid vector pCG806 - incomplete, maltose binding.
KW   cloning vector; maltose binding fusion protein.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-63
RC   pPL-5A from malE gene
RC   pCG806 from pUC18 & pmalE, malE gene
RC   pCG150 from pUC18 & pPL-5A, malE gene
RC   pCG325 from pCG150 & pMLB1034
RC   pPL-6A' from pHI28 & pmalE & pCH2
RC   pCG228 from pUC18 & pGW4400
RC   pCG410 from pCG228 & pCG150
RC   pCG828 from pCG228 & pCG806
RC   pCG1001 from pACYC184 & modification methylase
RA   Guan C.D., Li P., Riggs P.D., Inouye H.;
RT   "Vectors that facilitate the expression and purification of foreign
RT   peptides in Escherichia coli by fusion to maltose-binding protein";
RL   Gene 67:21-30(1988).
CC   NCBI gi: 208297
CC   NM (pCG806)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli K-12)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. malE gene/signal
FT                   -> pmalE
FT                   1. pUC18 SacI, MCS
FT                   2. pmalE HinfI-HinfI 1200bp, malE gene/signal
FT                   fill in:fill in
FT                   -> pCG806"
FT   misc_recomb     3..4
FT                   /note="E. coli malE end/E. coli lacZ start"
FT   CDS             <1..>63
FT                   /note="GEN E. coli fusion protein gene;
FT                   NCBI gi: 208298"
SQ   Sequence 63 BP; 14 A; 18 C; 18 G; 13 T; 0 other;
     cagactaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc