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ID   PCGN978    preliminary; circular DNA; SYN; 3540 BP.
AC   ATCC67064;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Higher plants/Agrobacterium/E.coli plasmid vector pCGN978 - incomplet.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCGN978 from RK2 & aroA gene
RA   ;
RT   ;
RL   Unpublished (1992).
RL   U.S. Patent No. 5,107,065 dated April 21(1992).
CC   Binary plasmid containing aroA in both sense (octopine synthetase or
CC   ocs promoter) and anti-sense (mas promoter) orientations.
CC   The presence of the anti-sense construct is effective in reducing aroA
CC   expression relative to controls containing the gene only in the sense
CC   orientation.
CC   USDA permit (APHIS form 2000) required for distribution. (ATCC staff)
CC   Restriction digests of the clone give the following sizes (kb):
CC   SalI--21.0, 8.8, 6.0; BglII--20.2; 15.0; EcoRI--22.0, 4.4, 4.0, 3.4,
CC   1.6; BamHI--25.6, 8.0, 1.6; ClaI--22.0, 6.5, 2.6, 2.4, 1.8.
CC   (ATCC staff)
CC   Medium is -1 1065 plus kanamycin (50 ug/ml) plus streptomcyin
CC   (20 ug/ml).
CC   NM (pCGN978)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Agrobacterium tumefaciens K12)(Agrobacterium tumefaciens)
CC   HO (E.coli)(higher plants)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. RK2, ori/cat gene/tet gene/kan gene
FT                   2. aroA gene
FT                   -> pCGN978"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;