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ID   PCHLORACEB preliminary; circular DNA; SYN; 4364 BP.
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AC   IG1054;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Eukaryote plasmid vector pChlorAce-B - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pChlorAce-B from pBR322
RA   ;
RT   ;
RL   Unpublished (1992).
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CC   NM (pChlorAce-B)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (USB)
CC   HO (E.coli)(COS cells)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pBR322)
CC   BR (pChlorAce-C)(pChlorAce-E)(pChlorAce-P)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> pChlorAce-B 4364bp"
FT   misc_binding    2242..2266
FT                   /note="MCS HindIII-SphI-PstI-SalI-AccI-XbaI"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cml/cmr)"
FT   misc_feature    2315..2315
FT                   /note="ATG"
FT   misc_binding    2528..2528
FT                   /note="SIT EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   misc_binding    3910..3910
FT                   /note="SIT BamHI"
FT   misc_binding    3920..3920
FT                   /note="SIT EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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