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ID   PCHR81     preliminary; circular DNA; SYN; 4000 BP.
AC   ATCC37535;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pCHR81 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCHR71 from R388
RC   pCHR81 from pCHR71 & Tn5, kan gene
RC   pCHR82 from pCHR71 & Tn5-TC1, tet gene
RC   pCHR83 from pCHR71 & Tn5-CM, cat gene
RC   pCHR84 from pCHR71 & Tn5-GM, GmR
RA   Sasakawa C., Yoshikawa M.;
RT   "A series of Tn5 variants with various drug-resistance markers and
RT   suicide vector for transposon mutagenesis";
RL   Gene 56:283-288(1987).
RN   [2]
RC   pUB5573 from R388
RC   pUB5554 from R388 & Tn10
RC   pUB5578 from S-a
RC   pUB1605 from pUB1604
RC   pUB1629 from pUB1605 & S-a
RC   pUB5579 from R7K
RC   R388, S-a, R7K from E.coli IncW plasmids
RA   Ward J.M., Grinsted J.;
RT   "Physical and genetic analysis of the Inc-W group plasmids R388,
RT   Sa, and R7K";
RL   Plasmid 7:239-250(1982).
RN   [3]
RC   pTH10 from RP4
RA   Harayama S., Tsuda M., Iino T.;
RT   "High frequency mobilization of the chromosome of Escherichia coli
RT   by a mutant of plasmid RP4 temperature-sensitive for maintenance";
RL   Mol. Gen. Genet. 180:47-56(1980).
RN   [4]
RC   pJB9JI from phage Mu & pPH1JI
RC   pJB4JI from pJB9JI & Tn5, kan gene
RA   Beringer J.E., Beynon J.L., Buchanan-Wollaston A.V., Johnston A.V.;
RT   "Transfer of the drug-resistance transposon Tn5 to Rhizobium";
RL   Nature 276:633-634(1978).
RN   [5]
RC   pGS9 from pCU101
RC   pGS12 from pRK248 & pACYC177
RC   pGS6 from pACYC177 & pCU101
RC   pGS27 from pGS6 & P1Cm, Tn9 cat gene
RC   [lambda::TnI from lambda & TnI]
RC   pGS18 from pCU101 & lambda::TnI
RA   Selvaraj G., Iyer V.N.;
RT   "Suicide plasmid vehicles for insertion mutagenesis in Rhizobium
RT   meliloti and related bacteria";
RL   J. Bacteriol. 156:1292-1300(1983).
RN   [6]
RC   R388, R409, R411 from E.coli incW plasmid
RC   R406 from K.aerogenes
RC   R389, R403, R404, R405, R407, R408, R410, R412, R413 from Klebsiella
RC   R413, R419, R420, R421, R422, R423, R424 from Klebsiella
RA   Datta N., Hedges R.W.;
RT   "Trimethoprim resistance conferred by W plasmid in
RT   Enterobacteriacea";
RL   J. Gen. Microbiol. 72:349-355(1972).
RN   [7]
RC   R7K from Proteus rettgeri
RC   R390, R391, R392, R394, R395, R396, R397, R398 from Proteus rettgeri
RC   R400, R401, R402 from Proteus rettgeri
RA   Coetzee J.N., Datta N., Hedges R.W.;
RT   "R factors from Proteus rettgeri";
RL   J. Gen. Microbiol. 72:543-552(1972).
RN   [8]
RC   p360/1 from R388 & RP1, TnA or Tn801
RC   p351/3 from R388 & RP1, TnA or Tn801
RC   pUB5501 from p351/3
RC   pUB5502 from p351/3
RC   pUB5520 from p360/1
RA   Ward J.M., Grinsted J.;
RT   "Mapping of functions in the R-plasmid R388 by examination of
RT   deletion mutants generated in vitro";
RL   Gene 3:87-95(1978).
RN   [9]
RC   pUB1604 from R388 & RP1, TnA or Tn801
RC   pUB1645 from R388 & RP1, TnA or Tn801
RC   pUB1646 from R388 & RP1, TnA or Tn801
RC   pUB1647 from R388 & RP1, TnA or Tn801
RA   Wallace L.J.;
RT   ;
RL   Unpublished (1982).
RN   [10]
RC   pCU1 from E.coli IncN plasmids
RA   Konarska-Kozlowska M., Iyer V.N.;
RT   "Physical and genetic organization of the IncN-group plasmid pCU1";
RL   Gene 14:195-204(1981).
RN   [11]
RC   pCU29 from pCU1 & Tn5
RC   pCU101 from pCU29 & pACYC184
RA   Thatte V., Iyer V.N.;
RT   "Cloning of a plasmid region specifying the N transfer system of
RT   bacterial conjugation in Escherichia coli";
RL   Gene 21:227-236(1983).
RN   [12]
RC   pCU51 from pCU29
RA   Thatte V.;
RT   ;
RL   Unpublished (1983).
RN   [13]
RC   pGS33 from pACYC184 & tet gene
RC   pGS38 from pGS33 & Tn5
RA   Iyer V.N.;
RT   ;
RL   Unpublished (1983).
RN   [14]
RC   pJB4JI from pJB9JI & Tn5
RA   Ely B.;
RT   "Transfer of drug resistance factors to the dimorphic bacterium
RT   Caulobacter crescentus";
RL   Genetics 91:371-380(1979).
CC   Replication is temperature-sensitive.
CC   Constructed by mutagenizing R388 to construct a temperature sensitive
CC   derivative and then transposing on Tn5.
CC   Can be used as a suicide vector for the delivery of transposable
CC   elements.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--32, 4.0, 1.8; PstI--36, 2.7, 1.8; HindIII--38, 3.8, 3.0. (ATCC
CC   staff)
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pCHR81)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli MC1061)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. E.coli IncW plasmids, sulf/tpr genes
FT                   -> R388 34000bp
FT                   1. R388 BglII-BglII 9000bp, ori/tpr gene
FT                   mutagenesis to thermosensitive ori
FT                   2. R388 remove BglII-BglII 9000bp, ori/tpr gene
FT                   -> pCHR71
FT                   1. pCHR71
FT                   transposition
FT                   2. Tn5 IS50L-IS50R 5818bp, kan gene
FT                   -> pCHR81 4000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli R388"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli tmpR gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;