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ID   PCHR83     preliminary; circular DNA; SYN; 3800 BP.
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AC   ATCC37537;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCHR83 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCHR71 from R388
RC   pCHR81 from pCHR71 & Tn5, kan gene
RC   pCHR82 from pCHR71 & Tn5-TC1, tet gene
RC   pCHR83 from pCHR71 & Tn5-CM, cat gene
RC   pCHR84 from pCHR71 & Tn5-GM, GmR
RA   Sasakawa C., Yoshikawa M.;
RT   "A series of Tn5 variants with various drug-resistance markers and
RT   suicide vector for transposon mutagenesis";
RL   Gene 56:283-288(1987).
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CC   Replication is temperature sensitive.
CC   Useful for transposon mutagenesis.
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pCHR83)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli MC1061)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E.coli IncW plasmids
FT                   -> R388 34000bp
FT                   1. R388 BglII-BglII 9000bp, ori/tpr gene
FT                   mutagenesis to thermosensitive ori
FT                   2. R388 remove BglII-BglII 9000bp, ori/tpr gene
FT                   -> pCHR71
FT                   1. pCHR71
FT                   transposition
FT                   2. Tn5-CM IS50L-IS50R 5818bp, cat gene
FT                   -> pCHR83 3800bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli R388"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT E. coli tmpR gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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