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ID   PCLII      preliminary; circular DNA; SYN; 1 BP.
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AC   IG7009;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCLII - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pLK5 from lambda, pR/cro gene
RC   pCL8, pCL23, pCL27, pCL47, pCL72 from pLG400 & pLK5
RC   pCLI, pCL type I series from pLG400 & pLK5
RC   pCL6, pCL19, pCLII, pCL type II series from pLG400 & pLK5
RC   pBRT12 from pBR322 & fd terminator
RA   Zabeau M., Stanley K.K.;
RT   "Enhanced expression of cro-beta-galactosidase fusion proteins
RT   under the control of the PR promoter of bacteriophage lambda";
RL   EMBO J. 1:1217-1224(1982).
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CC   NM (pCLII)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pCL)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 180bp, pR/cro gene
FT                   -> pLK5
FT                   1. pLG400 HindIII, before lacZ gene
FT                   fill in:fill in
FT                   remove small PstI-PstI, 3' amp gene
FT                   2. pLK5 BamHI, 350 bp 3' to cro gene
FT                   BAL31 nuclease
FT                   PstI-PstI 830bp-930bp, pR/cro gene
FT                   -> pCLII [class II, BamHI, no HindIII]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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