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ID   PCLL34     preliminary; circular DNA; SYN; 19800 BP.
AC   IG5091; ATCC67346;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Streptomyces plasmid vector pCLL34 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCLL34 from pIJ486 & pARC1, SLP1.2 ori
RA   Feitelson J.S.;
RT   "An improved plasmid for the isolation and analysis of Streptomyces
RT   promoters";
RL   Gene 66:159-162(1988).
RN   [2]
RC   pCLL34 from pIJ486 & pARC1, SLP1.2 ori
RA   ;
RT   ;
RL   Unpublished (1991).
RL   U.S. Patent No. 4,994,389 dated Feb. 19, 1991.
CC   Promoter-cloning vector permitting visual detection of activity by
CC   expression of a brown pigment. pCLL34 is a promoter-cloning vector
CC   derived from pARC1 by the insertion into the BamHI site of a 844 bp
CC   BclI fragment from pIJ486 containing the fd terminator and a
CC   polylinker.
CC   Constructed by inserting a 844 bp BclI fragment from pIJ486 containing
CC   the fd terminator and a polylinker into the BamHI site of pARC1. The
CC   additional 35 bp of MCS between the BamHI site and the start of the
CC   brown genes do not interfere with gene expression. Restriction digests
CC   of the clone give the following sizes (kb): EcoRI--16.71, 3.41. The
CC   order of the major features in this plasmid is: fd terminator - MCS -
CC   brown genes - tsrR. (U.S. Patent No. 4,994,389 dated Feb. 19, 1991)
CC   Note: This material is cited in a U.S and/or other Patent and may not
CC   be used to infringe the patent claims.
CC   Deposited by: American Cyanamid
CC   Growth media: R2 YE medium (ATCC medium number 1339)
CC   MCS. not original sequence.
CC   NM (pCLL34)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)(American Cyanamid)
CC   HO (Streptomyces)
CC   CP ()
CC   FN (cloning)(promoter-cloning)
CC   SE ()
CC   PA (pARC1)(pIJ486)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. SLP1.2, ori/tsr/thio
FT                   -> pARC1 19000bp
FT                   1. pIJ486 BclI-BclI 844bp, fd term
FT                   2. pARC1 BamHI-BamHI, SLP1.2 ori/tsr/thio
FT                   calf intestinal alkaline phosphatase
FT                   -> pCLL34 19800bp"
FT   CDS             0..0
FT                   /note="ANT tsr gene"
FT   terminator      0..0
FT                   /note="TER phage fd"
FT   misc_binding    0..0
FT                   /note="MCS BglII-PstI-HindIII-SphI-PstI-SalI-XbaI-
FT                   BamHI-SmaI-KpnI-SstI-EcoRI-BglII-BclI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 83 BP; 19 A; 26 C; 21 G; 17 T; 0 other;
     agatctgcag ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
     ctcgaattcc ccagatctga tca