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ID   PCLL9      preliminary; circular DNA; SYN; 9910 BP.
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AC   ATCC67449;
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DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Actinomadura kijaniata/S.lividans/E.coli plasmid pCLL9 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pAkij1 from Actinomadura kijaniata
RC   pCLL9 from pAkij1 & E. coli amp/tsrR/thio
RA   ;
RT   ;
RL   Unpublished (1991).
RL   U.S. Patent No. 5,002,891 dated Mar. 26, 1991.
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CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--10.2; BglII--10.2; PstI--10.2. (ATCC staff)
CC   Cloning into the PstI site inactivates the ampicillin marker used
CC   for selection in Escherichia coli. Cloning into the BglII, SphI, or
CC   SstI sites inactivates the tyrosinase gene.
CC   The pAkij1 ori may be useful in other taxa such as
CC   Bacillus, Staphylococcus and other Actinomycetes, including
CC   Streptosporangium, Actinoplanes, Nocardia, and Micromonospora. [1]
CC   Note: This material is cited in a U.S and/or other Patent and may not
CC   be used to infringe the patent claims.
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   NM (pCLL9)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)(American Cyanamid Company)
CC   HO (E.coli)(E.coli W5445)(Actinomadura kijaniata)
CC   HO (Streptomyces lividans)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE (mel tyrosinase)
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Actinomadura kijaniata
FT                   -> pAkij1
FT                   1. pAkij1
FT                   2. pBR322 800bp, amp gene
FT                   3. Streptomyces, tsrR/thio gene
FT                   -> pCLL9 9910bp"
FT   rep_origin      0..0
FT                   /note="ORI Actinomadura kijaniata pAkij1"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT Streptomyces tsrR/thio gene"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT SphI"
FT   misc_binding    0..0
FT                   /note="SIT SstI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//