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ID   PCMVADH    preliminary; circular DNA; SYN; 9000 BP.
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AC   ATCC77168;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pCMVADH - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRSVADH from pWX008 & pRc/RSV
RC   pRSVPAP from pSV2Apap & pRc/RSV
RC   plasmid from pM-MuLV-SV-lacZ & pSK+
RC   plasmid from plasmid & pECE
RC   pRSVlacZ from plasmid & pRc/RSV
RC   pCMVlacZ from pM-MuLV-SV-lacZ
RC   pCMVADH from pWX008
RA   Lin W.C., Culp L.A.;
RT   "Selectable plasmid vectors with alternative and ultrasensitive
RT   histochemical marker genes";
RL   Biotechniques 11:344-351(1991).
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RN   [2]
RC   pNS1191 from pUC118 & pUC119 & Drosophila ADH gene
RC   pWX4063 from pNS1191 & pUC18
RC   pWX4064 from pNS1191 & pUC18
RC   pNS1186 from pNS1191 & pUC118
RC   pNSdelta1 from pNS1191
RC   pNS16 from pWX1019 & pNSdelta1
RC   pWX4009, pWX4014, pWX4015, pWX4016, pWX4017 from pNS1191 & pUC18
RC   pWX008 from Drosophila alcohol dehydrogenase gene
RA   Shen N.L., Subrahmanyam G., Clark W., Martin P., Sofer W.;
RT   "Analysis of Adh gene regulation in Drosophila: studies using
RT   somatic transformation";
RL   Dev. Genet. 10:210-219(1989).
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RN   [3]
RC   pWX1001, pWX1002 from Carnegie 4 & Drosophila ADH gene
RC   pWX1003, pWX1004 from Carnegie 4 & Drosophila ADH gene
RC   pWX1005, pWX1006 from Carnegie 4 & Drosophila ADH gene
RC   pWX1007, pWX1008 from Carnegie 4 & Drosophila ADH gene
RC   pWX1009, pWX1010 from Carnegie 4 & Drosophila ADH gene
RC   pWX1011, pWX1012 from pUC18 & pSAF-II, Drosophila ADH gene
RC   pWX1013, pWX1014 from pUC18 & pSAF-II, Drosophila ADH gene
RC   pWX1015, pWX1016 from pUC18 & pSAF-II, Drosophila ADH gene
RC   pWX1017 from pUC18 & pSAF-II, Drosophila ADH gene
RC   pWX1019, pWX1020 from pUC18 & pSAF-II, Drosophila ADH gene
RC   psAC1 from pBR322 & Drosophila ADH gene
RC   psAF2 from pSV2 & Drosophila ADH gene
RC   lambda-17.1 from lambda & Drosophila ADH gene
RA   Martin P.F., Martin A., Osmani A., Sofer W.;
RT   "A transient expression assay for tissue-specific gene expression
RT   of alcohol dehydrogenase in Drosophila";
RL   Dev. Biol. 117:574-580(1986).
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RN   [4]
RC   from pBR322 & Drosophila alcohol dehydrogenase gene
RA   Sofer W., Martin P.F.;
RT   "Analysis of alcohol dehydrogenase gene expression in Drosophila";
RL   Annu. Rev. Genet. 21:203-225(1987).
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RN   [5]
RC   pSAF-II from Drosophila alcohol dehydrogenase gene
RA   Goldberg D.A.;
RT   "Isolation and partial characterization of the alcohol dehydrogenase
RT   gene";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:5794-5798(1980).
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CC   Shuttle reporter plasmid permitting visual detection of activity by
CC   histochemical staining.
CC   Presence of alcohol dehydrogenase activity is used to follow cell
CC   lineage in culture or in situ.
CC   The order of the major features in this plasmid is:CMV promoter -
CC   Drosophila ADH-s - SV40 polyadenylation signal - RSV promoter - neoR -
CC   polyoma ori - SV40 ori - pMB1 ori. (personal communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--9.2; HindIII/XbaI--7.2, 1.8; SmaI--9.2; StuI--9.2. (ATCC staff)
CC   Medium is 1799 LB plus neomycin (10 ug/ml).
CC   NM (pCMVADH)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli DH5alpha)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Drosophila ADH-s gene
FT                   -> pWX008
FT                   1. pWX008 HindIII-XbaI 1800bp, Drosophila ADH-s gene
FT                   2. pRc/CMV large XbaI-HindIII
FT                   -> pCMVADH 9000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   rep_origin      0..0
FT                   /note="ORI polyoma virus"
FT   promoter        0..0
FT                   /note="PRO CMV genes"
FT   promoter        0..0
FT                   /note="PRO RSV genes"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//