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ID   PCMVLACZII preliminary; circular DNA; SYN; 10900 BP.
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AC   ATCC77169;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pCMVlacZII - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRSVADH from pWX008 & pRc/RSV
RC   pRSVPAP from pSV2Apap & pRc/RSV
RC   plasmid from pM-MuLV-SV-lacZ & pSK+
RC   plasmid from plasmid & pECE
RC   pRSVlacZ from plasmid & pRc/RSV
RC   pCMVlacZ from pM-MuLV-SV-lacZ
RC   pCMVADH from pWX008
RA   Lin W.C., Culp L.A.;
RT   "Selectable plasmid vectors with alternative and ultrasensitive
RT   histochemical marker genes";
RL   Biotechniques 11:344-351(1991).
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RN   [2]
RC   pMBL524 from E.coli lacZ gene
RC   pM-MuLV-SV-lacZ from pB6 & pCH110-3-SVE & pMBL524, E.coli lacZ gene
RA   Sanes J.R., Rubenstein J.L., Nicolas J.F.;
RT   "Use of a recombinant retrovirus to study post-implantation cell
RT   lineage in mouse embryos";
RL   EMBO J. 5:3133-3142(1986).
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RN   [3]
RC   pB6 from MMLV provirus genome
RA   Yermanian P.;
RT   ;
RL   Thesis [Univ. Paris XII] 0:0-0(1984).
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RN   [4]
RC   pCH110-3-SVE from pCH110, SV40 promoter/lacZ
RA   Herbomel P.;
RT   ;
RL   Unpublished (1986).
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RN   [5]
RC   pMLB524 from pBR322 & lacZ gene
RC   pIP4, pIP5 from pMLB524
RA   Parsot C., Saint-Girons I., Cossard P.;
RT   "DNA sequence change of a deletion mutation abolishing attenuation
RT   control of the threonine operon of E. coli K12";
RL   Mol. Gen. Genet. 188:455-458(1982).
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RN   [6]
RC   pMLB524 from pBR322 & lacZ gene
RA   Berman M.L., Fowler A., Zabin I., Christensen L., Fiil N.P.,
RA   Hall M.N.;
RT   "Use of gene fusions for clonign and sequence analysis of the ompF
RT   gene of Escherichia coli";
RL   Unpublished (1982).
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RN   [7]
RC   pRcRSV from RSV
RC   PECE from RSV
RC   pSK+ from RSV
RA   Kung H.J.;
RT   ;
RL   Unpublished (1991).
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CC   Shuttle reporter plasmid permitting visual detection of activity by
CC   histochemical staining.
CC   Presence of beta-galactosidase activity is used to follow cell lineage
CC   in culture or in situ.
CC   The order of the major features in this plasmid is:CMV promoter -
CC   bacterial lacZ - polyadenylation signal - RSV promoter - neoR -
CC   polyoma virus ori - SV40 ori - pMB1 ori - M13 ori. (personal
CC   communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--9.6, 1.3; HindIII/XbaI--7.2, 3.8; KpnI--7.4, 3.6; StuI--11.0.
CC   (ATCC staff)
CC   Medium is 1799 LB plus neomycin (10 ug/ml).
CC   NM (pCMVlacZII)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli DH5alpha)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. MMLV provirus genome
FT                   2. pBR322
FT                   -> pB6
FT                   1. pCH110 PvuII, 5' to SV40 promoter/lacZ
FT                   SalI linker
FT                   -> pCH110-3-SVE
FT                   1. pBR322 remove EcoRI-AvaI, tet gene
FT                   2. E.coli EcoRI- , 5' lactose permease gene
FT                   3. E.coli -AvaI, 3' lacZ gene
FT                   -> pMBL524 3521bp
FT                   1. pB6 SalI-BamHI 8200bp
FT                   2. pCH110-3-SVE SalI-EcoRI 3600bp, SV40 prom/lacZ gene
FT                   3. pMBL524 AvaI, E.coli lacZ gene
FT                   BamHI linker
FT                   BamHI-EcoRI 320bp, E.coli lacZ gene 3'
FT                   -> pM-MuLV-SV-lacZ
FT                   1. pM-MuLV-SV-lacZ KpnI-BamHI 3700bp, lacZ gene
FT                   2. pSK+ remove small KpnI-BamHI
FT                   -> plasmid [3' XbaI]
FT                   1. plasmid KpnI-XbaI, lacZ gene
FT                   2. pECE remove small KpnI-XbaI
FT                   -> plasmid2 [5' HindIII]
FT                   1. plasmid2 HindIII-XbaI, lacZ gene
FT                   2. pRc/CMV remove small HindIII-XbaI
FT                   -> pCMVlacZII 10900bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   rep_origin      0..0
FT                   /note="ORI polyoma virus"
FT   promoter        0..0
FT                   /note="PRO CMV genes"
FT   promoter        0..0
FT                   /note="PRO RSV genes"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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