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ID   PCN39      preliminary; circular DNA; SYN; 4100 BP.
AC   ATCC77096;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Pseudomonas plasmid vector pCN39 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pPS10 from P.savastanoi
RC   pCN38 from pPS10 & pKT231
RC   pCN39 from pCN38 & pUC7
RC   pCN40 from pCN39 & pKT231
RC   pCN41 from pCN40
RC   pCN50 from pCN38 & pBR322
RC   pCN51 from pCN50
RC   pCN60 from pUC18 & pCN40
RC   pCN61 from pCN60
RC   pNSV1 from pCN38 & pCP3
RA   Nieto C., Fernandez-Tresguerres E., Sanchez N., Vicente M., Diaz R.;
RT   "Cloning vectors, derived from a naturally occurring plasmid of
RT   Pseudomonas savastanoi, specifically tailored for genetic
RT   manipulations in Pseudomonas";
RL   Gene 87:145-149(1990).
CC   Constructed by inserting a 0.9 kb PstI fragment from R1 into the PstI
CC   site of pUC7, cloning a 0.9 kb BamHI fragment from this intermediate
CC   into the BamHI site of pCN38 (ATCC 77095), and deleting a 0.9 kb PstI
CC   fragment. Contains the basic replicon of pPS10 which has been
CC   sequenced (EMBL accession number X58896).  The repA portion is
CC   flanked by EcoRI sites (nt 536 & 1500), with the site at nt 536
CC   between the repA promoter and coding sequences. (personal
CC   communication)
CC   Generates the equivalent of pCN38 with part of the multiple cloning
CC   site of pUC7.
CC   General purpose vector. (ATCC staff)
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.4; KpnI/PvuII--2.6, 1.6; HindIII/PstI--2.8, 1.4. (ATCC staff)
CC   The order of the major features in this plasmid is:PvuII - MCS - kanR
CC   - HindIII - KpnI - repA.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pCN39)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Pseudomonas aeruginosa PAO1024 [ATCC 47053])(Pseudomonas)
CC   HO (P.aeruginosa PAO1162)(P.putida KT2440 [ATCC 47054])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC7 PstI 2674bp 256..256
FT                   2. R1 PstI-PstI 1400bp, ori
FT                   -> plasmid 4100bp
FT                   1. plasmid BamHI-BamHI 900bp, R1 ori
FT                   2. pCN38 BamHI 4100bp, P.savastanoi
FT                   -> plasmid2 5000bp
FT                   1. plasmid2 remove PstI-PstI 900bp, R1 ori
FT                   -> pCN39 4100bp"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-SmaI-PstI-SmaI-BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI-BamHI-SmaI-PstI-PvuII"
FT   rep_origin      0..0
FT                   /note="ORI Pseudomonas pPS10; incompatibility groups
FT                   P1, P4/Q and WrepA"
FT   CDS             0..0
FT                   /note="ANT Pseudomonas kanamycin resistance gene
FT                   (kan)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;