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ID   PCN40      preliminary; circular DNA; SYN; 6000 BP.
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AC   ATCC77097;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Pseudomonas plasmid vector pCN40 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pPS10 from P.savastanoi
RC   pCN38 from pPS10 & pKT231
RC   pCN39 from pCN38 & pUC7
RC   pCN40 from pCN39 & pKT231
RC   pCN41 from pCN40
RC   pCN50 from pCN38 & pBR322
RC   pCN51 from pCN50
RC   pCN60 from pUC18 & pCN40
RC   pCN61 from pCN60
RC   pNSV1 from pCN38 & pCP3
RA   Nieto C., Fernandez-Tresguerres E., Sanchez N., Vicente M., Diaz R.;
RT   "Cloning vectors, derived from a naturally occurring plasmid of
RT   Pseudomonas savastanoi, specifically tailored for genetic
RT   manipulations in Pseudomonas";
RL   Gene 87:145-149(1990).
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CC   Constructed by replacing the PvuII/PstI fragment of pCN39 (ATCC 77096)
CC   with a 1.9 kb PvuII/PstI fragment of pKT231 encoding streptomycin
CC   resistance.
CC   Contains the basic replicon of pPS10 which has been sequenced (EMBL
CC   accession number X58896).  The repA portion is flanked by EcoRI sites
CC   (nt 536 & 1500), with the site at nt 536 between the repA promoter and
CC   coding sequences. (personal communication)
CC   The kanR gene can be insertionally inactivated by HindIII digestion.
CC   General purpose vector. (ATCC staff)
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--6.0; PstI/PvuII--4.1, 1.9; KpnI/SmaI--4.9, 1.1; SmaI--6.0.
CC   (ATCC staff)
CC   The order of the major features in this plasmid is:kanR - PvuII - strR
CC   - PstI - SmaI - BamHI - kanR - HindIII.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pCN40)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Pseudomonas aeruginosa PAO1024 [ATCC 47053])(Pseudomonas)
CC   HO (P.aeruginosa PAO1162)(P.putida KT2440 [ATCC 47054])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pCN39 remove PvuII-PstI
FT                   2. pKT231 PvuII-PStI 1900bp, spec gene
FT                   -> pCN40 6000bp"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-SmaI-PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI-HindIII-BamHI-SmaI-PstI-PvuII"
FT   rep_origin      0..0
FT                   /note="ORI Pseudomonas pPS10; incompatibility groups
FT                   P1, P4/Q and WrepA"
FT   CDS             0..0
FT                   /note="ANT Pseudomonas kanamycin resistance gene
FT                   (kan)"
FT   CDS             0..0
FT                   /note="ANT Pseudomonas streptomycin resistance gene
FT                   (str)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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