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ID   PCOLIR515  preliminary; circular DNA; SYN; 72 BP.
AC   M31317;
DT   15-JUN-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pColIR515 - incomplete, pBR322 deletion.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-72
RC   pPS11 from pUC18 & pHD127-16
RC   pColIR515 from pBR322 & ColE1
RA   Scholten P.M., Nordheim A.;
RT   "Diethyl pyrocarbonate: A chemical probe for DNA cruciforms";
RL   Nucleic Acids Res. 14:3981-3993(1986).
RN   [2]
RC   pColIR515 from pColIR215
RA   Lilley D.M., Hallam L.R.;
RT   "The interactions of enzyme and chemical probes with inverted
RT   repeats in supercoiled DNA";
RL   J. Biomol. Struc. Dyn. 1:169-180(1983).
RN   [3]
RC   pHD01-14 from pBR322 & E. coli gal operon
RC   pHD101-3 from pBR322 & pHD01-14
RC   pHD101A-3 from pHD101-3
RC   plasmid from pBR322 & oligo
RC   pHD127-16 from plasmid & pHD101-3
RA   Kemper B., Jensch F., Depka-Prondzynski M.V., Fritz H.J.,
RA   Borgmeyer U., Mizuuchi K.;
RT   "Resolution of Holliday structures by endonuclease VII as observed
RT   in interactions with cruciform DNA";
RL   Cold Spring Harb. Symp. Quant. Biol. 49:815-825(1984).
RN   [4]
RC   pColIR215 from pBR322 & ColE1
RC   pIRbke8 from pAT153 & oligo
RC   pDS1118 from pDS series
RA   Lilley D.M.;
RT   "Hairpin-loop formation by inverted repeats in supercoiled DNA is
RT   a local and transmissible property";
RL   Nucleic Acids Res. 9:1271-1289(1981).
CC   NCBI gi: 208962
CC   NM (pColIR515)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-BamHI 377bp
FT                   \ 4360..4361..376, tet 3984bp
FT                   2. ColE1 ?-? 442bp, inverted repeat
FT                   -> pColIR215 4427bp
FT                   1. pColIR215 remove BamHI-PvuII 1688bp,
FT                   \ pBR322 376..2067
FT                   -> pColIR515 2735bp"
FT   rep_origin      0..0
FT                   /note="ORI"
SQ   Sequence 72 BP; 22 A; 15 C; 13 G; 22 T; 0 other;
     ccaagcttat cgatgataag ctgtcaaaca tgagaattct catgtttgac agcttatcat
     cgataagctt gc