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ID   PCOS1EMBL  preliminary; circular DNA; SYN; 6300 BP.
AC   M18133; ATCC37568;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli cosmid vector pcos1EMBL - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pRK419lac from pRK419 & pAD3
RC   pcos4EMBLlac from pcos4EMBL & pAD3
RC   pcos1EMBL from pcos4EMBLlac & pRK419lac
RC   cos793 from pcos1EMBL
RC   cos798 from cos793
RC   cosU800 from pcos1EMBL & pUC9
RC   cos2801 from cos798 & cosU800
RC   pcos2EMBL from cos2801
RC   cos786 from pcos1EMBL & pGD3
RC   cos790 from cos786
RC   cos806 from cos790
RC   cos807 from cos806
RC   cos808 from cos807
RC   cos809 from cos808 & pcos2EMBL
RC   cos810K from cos809
RC   pcos3EMBL from cos810K
RC   cos851 from pcos3EMBL & pUC2
RC   cos852 from cos851
RC   pUC7deltaSal from pUC7
RC   cos854 from cos852 & pUC7deltaSal
RC   pcos5EMBL from cos854
RC   pM1380 from pir41 gene
RC   cos860 from pcos5EMBL & pM1380, pir41 gene
RC   pcos6EMBL from cos860
RC   pEMBL18-Not from R6K
RA   Ehrich E., Craig A., Poustka A., Frischauf A.M., Lehrach H.;
RT   "A family of cosmid vectors with the multi-copy R6K replication
RT   origin";
RL   Gene 57:229-237(1987).
RN   [2]
RC   pcos4EMBL or tetracos from lambda & pBR322, tet gene
RA   Poustka A., Brady G., Schutz G., Lehrach H.;
RT   ;
RL   Unpublished (1987).
RN   [3]
RC   pAD3 from pUC9
RA   Schaller H.;
RT   ;
RL   Unpublished (1987).
RN   [4]
RC   pGD3 from polyoma
RA   Colantuoni V.;
RT   ;
RL   Unpublished (1987).
RN   [5]
RC   pGN7 from R6K
RC   pGN7cop41, pGN7cop50 from pGN7
RC   pMI7 from pGN7cop41
RC   pMI370 from pGN7 & linker & pACYC184
RC   pMI380 from pGN7cop41 & linker & pACYC184
RC   pMI400 from pMC1403 & pMI370
RA   Inuzuka M., Wada Y.;
RT   "A single amino acid alteration in the initiation protein is
RT   responsible for the DNA overproduction phenotype of copy number
RT   mutants of plasmid R6K";
RL   EMBO J. 4:2301-2307(1985).
RN   [6]
RC   from pcos1EMBL
RC   from pcos2EMBL
RC   ps1EMBL from pRK419 & pUC8
RA   Poustka A., Rackwitz H.R., Frischauf A.M., Hohn B., Lehrach H.;
RT   "Selective isolation of cosmid clones by homologous recombination
RT   in Eschercichia coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:4129-4133(1984).
RN   [7]
RC   pRK43, pRK44 from RSF2124 & R6K
RC   pRK45 from pMB9 & R6K
RA   Kolter R., Helinski D.R.;
RT   "Activity of the replication terminus of plasmid R6K in hybrid
RT   replicons in Escherichia coli";
RL   J. Mol. Biol. 124:425-441(1978).
RN   [8]
RC   pDF38 from pML2 & trpE gene
RC   R6Kdelta1 from R6K
RA   Figurski D.;
RT   ;
RL   Unpublished (1978).
CC   One of a family of cosmid vectors (ATCC 37568-37571) containing a R6K
CC   origin.
CC   Contains one lambda cos site.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.3; SalI--6.3. (ATCC staff)
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pcos1EMBL)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli DH1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC9 2665bp, 3' to lacZ
FT                   2. linker BamHI-EcoRI 12bp ggatccgaattc
FT                   -> pAD3 2677bp [BamHI-EcoRI-lacZ-EcoRI-BamHI]
FT                   1. R6K, beta ori/gamma ori/kan gene
FT                   -> pRK419
FT                   1. pRK419 Sau3A/MboI
FT                   2. pAD3 BamHI-BamHI 254..?, EcoRI-lac-EcoRI
FT                   -> pRK419lac [EcoRI-lac-EcoRI]
FT                   1. pBR322 4361bp
FT                   2. lambda PvuII 212bp left..212, cos
FT                   3. lambda PvuII 212bp left..212, cos
FT                   -> pcos4EMBL 4785bp [2 tandem cos with PvuII between]
FT                   1. pcos4EMBL BglII 4785bp, lambda
FT                   2. pAD3 BamHI-BamHI 254..?, EcoRI-lac-EcoRI
FT                   -> pcos4EMBLlac
FT                   1. pcos4EMBLlac small EcoRI-EcoRI, cos/tet gene
FT                   2. pRK419lac EcoRI-lac-EcoRI, lacZ
FT                   -> pcos1EMBL 6300bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli R6K"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;