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ID   PCOS2EMBL  preliminary; circular DNA; SYN; 6100 BP.
AC   M18133; ATCC37569;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli cosmid vector pcos2EMBL - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pRK419lac from pRK419 & pAD3
RC   pcos4EMBLlac from pcos4EMBL & pAD3
RC   pcos1EMBL from pcos4EMBLlac & pRK419lac
RC   cos793 from pcos1EMBL
RC   cos798 from cos793
RC   cosU800 from pcos1EMBL & pUC9
RC   cos2801 from cos798 & cosU800
RC   pcos2EMBL from cos2801
RC   cos786 from pcos1EMBL & pGD3
RC   cos790 from cos786
RC   cos806 from cos790
RC   cos807 from cos806
RC   cos808 from cos807
RC   cos809 from cos808 & pcos2EMBL
RC   cos810K from cos809
RC   pcos3EMBL from cos810K
RC   cos851 from pcos3EMBL & pUC2
RC   cos852 from cos851
RC   pUC7deltaSal from pUC7
RC   cos854 from cos852 & pUC7deltaSal
RC   pcos5EMBL from cos854
RC   pM1380 from pir41 gene
RC   cos860 from pcos5EMBL & pM1380, pir41 gene
RC   pcos6EMBL from cos860
RC   pEMBL18-Not from R6K
RA   Ehrich E., Craig A., Poustka A., Frischauf A.M., Lehrach H.;
RT   "A family of cosmid vectors with the multi-copy R6K replication
RT   origin";
RL   Gene 57:229-237(1987).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.7; SalI--6.7; EcoRI--3.8, 3.0; BglII--5.2, 1.1, 0.55.
CC   (ATCC staff)
CC   Differs from pcos1EMBL (ATCC 37568) in having 2 lambda cos sites and
CC   a deletion in the region of the kanR gene.
CC   One of a family of cosmid vectors (ATCC 37568-37571) containing a R6K
CC   origin.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pcos2EMBL)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli DH1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pcos1EMBL remove EcoRI-PvuII, lambda cos
FT                   Klenow:
FT                   -> cos793 [EcoRI but no PvuII in cos]
FT                   1. cos793 SalI
FT                   fill in
FT                   -> cos798 [no tet]
FT                   1. pcos1EMBL EcoRI-EcoRI, cos/tet gene
FT                   2. pUC9 EcoRI 2665bp 264..264
FT                   -> cosU800
FT                   1. cos798 BstEII-BamHI
FT                   Klenow:
FT                   2. cosU800 EcoRI-BamHI
FT                   Klenow:
FT                   -> cos2801
FT                   1. cos2801 remove small XhoI-XhoI
FT                   -> pcos2EMBL 6100bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli R6K"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;