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ID   PCOS4EMBL  preliminary; circular DNA; SYN; 5200 BP.
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AC   IG9864;
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DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli cosmid vector pcos4EMBL - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pcos4EMBL from lambda, cos
RA   Reedy X., Lehrach H.;
RT   ;
RL   Unpublished (1983).
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CC   NM (pcos4EMBL)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP ()
CC   HO (E.coli DH5alpha)(E.coli)
CC   CP ()
CC   FN (cloning 35000-45000)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 PvuII 4361bp 2069..2069
FT                   2. lambda ?-PvuII 1000bp ?..leftend..212, 2 cos
FT                   3. lambda ?-PvuII 1000bp ?..leftend..212, 2 cos
FT                   -> pcos4EMBL 6300bp [2 tandem cos with PvuII between]
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//