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ID   PCOS5EMBL  preliminary; circular DNA; SYN; 6000 BP.
AC   M18133; ATCC37570;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli cosmid vector pcos5EMBL - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pRK419lac from pRK419 & pAD3
RC   pcos4EMBLlac from pcos4EMBL & pAD3
RC   pcos1EMBL from pcos4EMBLlac & pRK419lac
RC   cos793 from pcos1EMBL
RC   cos798 from cos793
RC   cosU800 from pcos1EMBL & pUC9
RC   cos2801 from cos798 & cosU800
RC   pcos2EMBL from cos2801
RC   cos786 from pcos1EMBL & pGD3
RC   cos790 from cos786
RC   cos806 from cos790
RC   cos807 from cos806
RC   cos808 from cos807
RC   cos809 from cos808 & pcos2EMBL
RC   cos810K from cos809
RC   pcos3EMBL from cos810K
RC   cos851 from pcos3EMBL & pUC2
RC   cos852 from cos851
RC   pUC7deltaSal from pUC7
RC   cos854 from cos852 & pUC7deltaSal
RC   pcos5EMBL from cos854
RC   pM1380 from pir41 gene
RC   cos860 from pcos5EMBL & pM1380, pir41 gene
RC   pcos6EMBL from cos860
RC   pEMBL18-Not from R6K
RA   Ehrich E., Craig A., Poustka A., Frischauf A.M., Lehrach H.;
RT   "A family of cosmid vectors with the multi-copy R6K replication
RT   origin";
RL   Gene 57:229-237(1987).
CC   Differs from pcos3EMBL in having NotI sites flanking the BamHI cloning
CC   site.  Total size is approx 6 kb.
CC   One of a family of cosmid vectors (ATCC 37568-37571) containing a R6K
CC   origin.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.0; EcoRI--6.0. (ATCC staff)
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pcos5EMBL)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli DH1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. polyoma
FT                   -> pGD3
FT                   1. pcos1EMBL BglII-BglII, tet/kan/cos
FT                   2. pGD3 BclI-BclI, polyoma
FT                   -> cos786
FT                   1. cos786 remove BamHI-BamHI, amp gene
FT                   -> cos790
FT                   1. cos790 remove SacI-SacI, polyoma
FT                   -> cos806
FT                   1. cos806 remove EcoRI-EcoRI, cos/tet gene
FT                   -> cos807
FT                   1. cos807 SacI-PvuII, polyoma
FT                   Klenow:
FT                   -> cos808
FT                   1. cos808 EcoRI
FT                   2. pcos2EMBL EcoRI-EcoRI, R6K ori/kan gene
FT                   -> cos809
FT                   1. cos809 remove EcoRI-BstEII, tet gene
FT                   :Klenow
FT                   -> cos810K
FT                   1. cos810K remove XhoI-XhoI, kan gene/6000bp
FT                   -> pcos3EMBL 6000bp [kan, 2 cos, 2 R6K, polyoma]
FT                   1. pcos3EMBL EcoRI 6000bp
FT                   Klenow
FT                   -> cos850 6000bp
FT                   1. cos850 BamHI 6000bp
FT                   2. pUC2 BglII 2200bp
FT                   -> cos851 8200bp [EcoRI, no BglII, no BamHI]
FT                   1. cos851 remove EcoRI-EcoRI 2700bp, pUC2/5500bp
FT                   -> cos852 5500bp
FT                   1. pUC7 SalI 2666bp 258..2674..246
FT                   fill in
FT                   -> pUC7deltaSal 2666bp
FT                   1. cos852 EcoRI 5500bp
FT                   EcoRI-NotI linker 14bp gaattcgcggccgc:NotI-EcoRI
FT                   \ linker 14bp gcggccgcgaattc
FT                   2. pUC7deltaSal ?HindIII 2666bp
FT                   -> cos854 8200bp
FT                   1. cos854 remove BamHI-BamHI 2200bp, pUC2/6000bp
FT                   -> pcos5EMBL 6000bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacII-NotI-BamHI-NotI-SacII-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli R6K"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;