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ID   PCP1       preliminary; circular DNA; SYN; 3600 BP.
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AC   S62800; ATCC37351;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pCP1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   plasmid from pUC9 & vaccinia virus 7.5kDa gene
RC   pCP1 from pCAT & plasmid
RC   pMM24 from pMM23 & SV40 72-bp repeats
RA   Cochran M.A., Mackett M., Moss B.;
RT   "Eukaryotic transient expression system dependent on transcription
RT   factors and regulatory DNA sequences of vaccinia virus";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:19-23(1985).
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RN   [2]
RC   plasmid from pBR328
RC   pGS8 from plasmid & vaccinia virus TK gene
RC   pMM1 from pUC9 & vaccinia virus TK gene
RC   pMM2 from pUC9 & vaccinia virus TK gene
RC   pMM3 from pMM1 & pMM2
RC   pMM4 from pMM3
RC   pMM5 from pMM4
RC   pGS15 from pUC9 & pAG4
RC   pGS19 from pGS15 & linker
RC   pGS20, pGS21 from pGS19 & pGS8
RC   [pGS30 from cat gene]
RC   pCAT from pBR328 & pUC7
RC   plasmid2 from pMM4 & pCAT
RC   plasmid3 from pGS20 & pCAT
RC   plasmid4 from pGS21 & pCAT
RC   vC1 from plasmid2 & vaccinia virus
RC   vC24 from plasmid2 & vaccinia virus
RC   vC30 from plasmid3 & vaccinia virus
RC   vC31 from plasmid3 & vaccinia virus
RA   Mackett M., Smith G.L., Moss B.;
RT   "General method for production and selection of infectious
RT   vaccinia virus recombinants expressing foreign genes";
RL   J. Virol. 49:857-864(1984).
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RN   [3]
RC   M13mp9-L5 from M13mp9 & vaccinia virus
RC   pMM22 from pBR328 & M13mp9-L5, vaccinia virus
RC   pLCAT1, pLCAT2 from pMM22 & M13mp9-L5, vaccinia late promoter
RC   pMM23 from pMM22 & pVP1
RC   vMM23 from pMM23
RC   vLCAT1 from pLCAT1
RC   vLCAT2 from pLCAT2
RA   Weir J.P., Moss B.;
RT   "Regulation of expression and nucleotide sequence of a late
RT   vaccinia virus gene";
RL   J. Virol. 51:662-669(1984).
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RN   [4]
RC   d174 from SV40 72-bp repeats
RA   Brady J., Radonovich M., Thoren M., Das G., Salzman N.P.;
RT   "Simian virus 40 major late promoter: an upstream DNA sequence
RT   required for efficient in vitro transcription";
RL   Mol. Cell. Biol. 4:133-141(1984).
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RN   [5]
RC   pAG4 from vaccinia virus 7.5K gene
RA   Venkatesan S., Moss B.;
RT   "In vitro transcription of the inverted terminal repetition of the
RT   vaccinia virus genome: correspondence of initiation and cap site";
RL   J. Virol. 37:738-747(1981).
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RN   [6]
RC   pVP1 from vaccinia virus 7.5K gene
RA   Mackett M., Smith G.L., Moss B.;
RT   "Vaccinia virus: a selectable eukaryotic cloning and expression
RT   vector";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:7415-7419(1982).
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CC   Transient expression plasmid for chloramphenicol acetyltransferase
CC   activity in cultured mammalian cells under the control of the vaccinia
CC   promoter.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCP1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(vertebrate cells)(E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR328 TaqI-TaqI 773bp 3448..4221, cat gene
FT                   2. pUC7 remove AccI-AccI 12bp 247..259, MCS/2662bp
FT                   -> pCAT 3435bp
FT                   1. pUC9 HincII 2665bp 250..250, MCS
FT                   2. vaccinia virus HincII-RsaI 135bp, 7.5 kDa gene
FT                   -> plasmid 2800bp
FT                   1. pCAT BamHI-BamHI 785bp, cat gene/pUC7 240..cat..264
FT                   2. plasmid BamHI 2800bp 254..254
FT                   -> pCP1 3600bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI-HindIII-HincII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO vaccinia genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 148 BP; 60 A; 27 C; 17 G; 44 T; 0 other;
     ctaattccaa acccacccgc tttttatagt aattttttca cgcataaata caataattaa
     tttctcgtaa aagtagaaaa tatattctaa cttattgcac ggtaaggaag tagaatcaga
     aagaacagta ctcaatcaat agcaatca
//