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ID   PCPOT      preliminary; circular DNA; SYN; 11700 BP.
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AC   ATCC39685;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pCPOT - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCPOT from yeast 2 micron & pBR322 & S.pombe POT1 gene
RA   ;
RT   ;
RL   Unpublished (1988).
RL   U.S. Patent No. 4,766,073 dated Aug. 23, 1988.
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RN   [2]
RC   pCPOT from yeast 2 micron & pBR322 & S.pombe POT1 gene
RA   ;
RT   ;
RL   Unpublished (1989).
RL   U.S. Patent No. 4,849,407 dated July 18, 1989.
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CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--14, 4.3; AccI--3.3, 2.7, 2.7, 2.5, 2.5, 2.2, 1.5, 1.3;
CC   BglI--11.0, 4.5, 2.5; EcoRI--4.2, 3.3, 2.9, 2.9, 1.7, 1.3, 1.3. (ATCC
CC   staff)
CC   The presence of the POT1 gene ensures stable maintenance of the
CC   plasmid in yeast during growth on nonselective medium using glucose
CC   as a carbon source. [1]
CC   An episomal Saccharomyces cerevisiae/E.coli shuttle expression
CC   vector containing 2 micron circle, pBR322 replication and selection,
CC   and Schizosaccharomyces pombe sequences encoding triose phosphate
CC   isomerase (POT1). [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCPOT)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast 2 micron, ori
FT                   3. S.pombe POT1 gene
FT                   4. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT                   -> pCPOT 11700bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SphI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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