ID   PCPP3      preliminary; circular DNA; SYN; 5600 BP.
XX
AC   ATCC37278;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/E.coli plasmid vector pCPP-3 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCPP-3 from pBR322 & pUB110 & pC194 & pK01-T
RC   pCPP-4 from pCPP-3 & linker
RA   Band L., Yansura D.G., Henner D.J.;
RT   "Construction of a vector for cloning promoters in Bacillus
RT   subtilis";
RL   Gene 26:313-315(1983).
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RN   [2]
RC   pK01-T from lambda, t0 terminator
RA   Rosenberg M.;
RT   ;
RL   Unpublished (1983).
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CC   For cloning promoters and determining activity based on
CC   chloramphenicol acetyltransferase expression.
CC   Medium is 1065 LB medium.
CC   NM (pCPP-3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli MM294)(Bacillus subtilis)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKO-1
FT                   2. lambda DpnI-DpnI 189bp 38478..38667, t0 terminator
FT                   -> pK01-T
FT                   1. pBR322 MboI/Sau3A-PvuII 1146bp 2067..3212,
FT                   \ MboI = 349 376 467 826 1098 1129 1144 1461 1668 3042
FT                   \ 3117 3128 3136 3214 3226 3331 3672 3690 3736 3994
FT                   \ 4011 4047
FT                   2. pUB110 PvuII-EcoRI 3514bp 1036..4548..2,
FT                   \ B.subtilis ori/neo gene
FT                   3. pC194 Sau3A/MboI-Sau3A/MboI 2004bp 1..2005, cat
FT                   4. pK01-T DpnI-DpnI 189bp 38478..38667,
FT                   \ lambda t0 terminator
FT                   -> pCPP-3 5600bp [unique BamHI]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-Sau3A-BamHI-PvuII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
XX
SQ   Sequence 22 BP; 8 A; 3 C; 6 G; 5 T; 0 other;
     gaattcggag gcatatcaaa tg
//