back Return to this vector's summary.
ID   PCPP4      preliminary; circular DNA; SYN; 5600 BP.
XX
AC   ATCC37277;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/E.coli plasmid vector pCPP-4 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pPCPP-3 from pBR322 & pUB110 & pC194 & pK01-T
RC   pPCPP-4 from pPCPP-3 & linker
RA   Band L., Yansura D.G., Henner D.J.;
RT   "Construction of a vector for cloning promoters in Bacillus
RT   subtilis";
RL   Gene 26:313-315(1983).
XX
CC   For cloning promoters and determining activity based on
CC   chloramphenicol acetyltransferase expression.
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pCPP-4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli MM294)(Bacillus subtilis)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPCPP-3 BamHI 5600bp, pUB110 789..789
FT                   fill in
FT                   -> plasmid 5600bp
FT                   1. plasmid EcoRI 5600bp, pUB110 2..2
FT                   BamHI linker 10bp aattggatcc
FT                   -> pPCPP-4 5600bp [unique BamHI before cat]"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-PvuII-Sau3A"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
XX
SQ   Sequence 32 BP; 11 A; 5 C; 8 G; 8 T; 0 other;
     gaattggatc caattcggag gcatatcaaa tg
//