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ID   PCQV2      preliminary; circular DNA; SYN; 46 BP.
AC   M19093;
DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Gram-negative bacteria plasmid vector pCQV2 - incomplete,phase-shift.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCQV0 from p1B1
RC   pCQV2 from pCQV0 & pBR322 & lambda, cI857 gene
RC   pBga12 from pCQV2 & lacZ gene
RC   p1A1 from pBR322 & lambda
RC   pCS1 from p1A1 & SV40 small t region
RC   pCS2 from pCS1 & pBR322
RC   pCS3 from pCS2
RC   pCQV3 from pCQV2 & pCS3
RC   pCQVS1 from pCQV3
RA   Queen C.;
RT   "A vector that uses phage signals for efficient synthesis of
RT   proteins in Escherichia coli";
RL   J. Mol. Appl. Genet. 2:1-10(1983).
RN   [2]
RC   lambda r32 from lambda
RC   p1B1 from pBR322 & lambda r32
RA   McKenney K., Rosenberg M.;
RT   ;
RL   Unpublished (1983).
CC   NCBI gi: 208162
CC   NM (pCQV2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Gram-negative bacteria)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda r32
FT                   1. pBR322 HindIII 4361bp 30..30
FT                   2. lambda r32 HindIII-HindIII 564bp 36896..37460,
FT                   \ 5' to cI to cI
FT                   \ lambda 23131 25158 27480 36896 37460 44142
FT                   -> p1B1 4925bp
FT                   1. p1B1 BglII 4925bp, lambda
FT                   BAL31 nuclease
FT                   BamHI-BamHI
FT                   Klenow:Klenow
FT                   -> pCQV0 [BamHI linked to ATG codon]
FT                   1. pCQV0 HgiAI-BamHI, cro/cII/O/P genes
FT                   2. pBR322 BamHI-ClaI 4010bp 376..4361..25
FT                   3. lambda cI857 TaqI-HgiAI, 5' to cI/pRM to 5' to pR
FT                   -> pCQV2 3000bp [ClaI]"
FT   rep_origin      0..0
FT                   /note="ORI"
SQ   Sequence 46 BP; 10 A; 11 C; 14 G; 11 T; 0 other;
     gtactaagga ggttgtatgg atcctctacg ccggacgcat gtcgac