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ID   PCRSCRIPT  preliminary; circular DNA; SYN; 1 BP.
AC   IG2050; X52328;
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phagemid vector pCR-Script SK(+) - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-2961
RC   pCR-Script SK(+)
RA   Thomas E.A.;
RT   ;
RL   Submitted (20-FEB-1990) on tape to the EMBL Data Library by:
RL   Thomas E.A., Stratagene Cloning Systems,
RL   11099 North Torrey Pines Rd., La Jolla, CA 92037, USA.
CC   The SK designation indicates the polylinker is oriented such
CC   that beta-galactosidase (lacZ) transcription proceeds through the SacI
CC   site first and the KpnI site last.
CC   pCR-Script SK(+) carries an F1 origin of replication,
CC   oriented such that transcription proceeds in
CC   the same direction as beta-galactosidase (lacZ) transcription.
CC   NM (pCR-Script SK+)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (coding)
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli)
CC   CP ()
CC   FN (mapping gene)(expression)
CC   SE (color blue/white)
CC   PA (pBluescript II SK+)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBluescribe 2746bp
FT                   2. oligo SacI-KpnI 20bp, MCS
FT                   -> pCR-Script SK(+) 2760bp"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="MCS SacI-SrfI/SmaI-KpnI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;