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ID PCS3 preliminary; circular DNA; SYN; 1 BP.
AC J01565; ATCC39142;
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE E. coli plasmid vector pCS3 - incomplete.
KW cloning vector.
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
RC pCS3 from pEW27
RC [pOP6 from pOP series]
RC pOP9 from pOP6 & pBR322
RA Wong E.M.;
RL Unpublished (1987).
RL U.S. Patent No. 4,677,064 dated June 30, 1987.
RC pEW27 from pBGP120
RC pMM1 from pNOP42
RC pEW2762 from pEW27
RC pMM700 from pKO-1 & pNOP42
RC pMM900 from pKO-1 & pNOP42
RC pMM727 from pKO-1 & pEW2762
RC pMM701 from pKO-1 & pMM1
RA Wong E.M., Muesing M.A., Polisky B.;
RT "Temperature-sensitive copy number mutants of CoIE1 are located in
RT an untranslated region of the plasmid genome";
RL Proc. Natl. Acad. Sci. U.S.A. 79:3570-3574(1982).
CC This contains the replication origin for high copy number at high
CC temperature of pEW27 (ATCC 37124). pOP9 was construced by cloning the
CC EcoRI/PvuII fragment containing the origin of pOP6 into pBR322.
CC This should NOT be grown over 30C; it is best between 28-30C.
CC Gives high copy number at high temperature.
CC Medium is 1227 LB plus ampicillin.
CC NM (pCS3)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli MM294)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
FH Key Location/Qualifiers
FT misc_feature 0..0
FT /note="1. pEW27 17299bp
FT -> pCS3 17000bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-PvuII-PstI-PvuI-ClaI-
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;