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ID   PCS3       preliminary; circular DNA; SYN; 1 BP.
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AC   J01565; ATCC39142;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pCS3 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCS3 from pEW27
RC   [pOP6 from pOP series]
RC   pOP9 from pOP6 & pBR322
RA   Wong E.M.;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,677,064 dated June 30, 1987.
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RN   [2]
RC   pEW27 from pBGP120
RC   pMM1 from pNOP42
RC   pEW2762 from pEW27
RC   pMM700 from pKO-1 & pNOP42
RC   pMM900 from pKO-1 & pNOP42
RC   pMM727 from pKO-1 & pEW2762
RC   pMM701 from pKO-1 & pMM1
RA   Wong E.M., Muesing M.A., Polisky B.;
RT   "Temperature-sensitive copy number mutants of CoIE1 are located in
RT   an untranslated region of the plasmid genome";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:3570-3574(1982).
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CC   This contains the replication origin for high copy number at high
CC   temperature of pEW27 (ATCC 37124). pOP9 was construced by cloning the
CC   EcoRI/PvuII fragment containing the origin of pOP6 into pBR322.
CC   This should NOT be grown over 30C; it is best between 28-30C.
CC   Gives high copy number at high temperature.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCS3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli MM294)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pEW27 17299bp
FT                   -> pCS3 17000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-PvuII-PstI-PvuI-ClaI-
FT                   HindIII-SphI-SalI-BamHI-NruI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
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