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ID   PCVD301    preliminary; circular DNA; SYN; 21500 BP.
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AC   ATCC37320;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Broad host range/E.coli cosmid vector pCVD301 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCVD300 from pRK290 & pREG153
RC   pCVD301 from M13mp7 & pCVD300
RC   pAD22 from pCVD301 & YRp7
RA   Datta A.R., Kaper J.B., MacQuillan A.M.;
RT   "Shuttle cloning vectors for the marine bacterium Vibrio
RT   parahaemolyticus";
RL   J. Bacteriol. 160:808-811(1984).
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CC   Broad host range cosmid vector which can be selected and replicate in
CC   E.coli and Vibrio parahaemolyticus.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pCVD301)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli JA300)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp
FT                   -> pREG153 10000bp
FT                   1. RK2
FT                   -> pRK290 20000bp
FT                   1. pRK290 BglII, tet gene/rix
FT                   2. pREG153 BglII-BglII, lambda cos
FT                   -> pCVD300 21500bp
FT                   1. M13mp7 EcoRI-EcoRI 42bp 6232..6274, MCS
FT                   2. pCVD300 EcoRI 21500bp
FT                   -> pCVD301 21500bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BamHI-SalI-PstI-SalI-BamHI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI-SalI-PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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