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ID   PCYM0      preliminary; circular DNA; SYN; 6360 BP.
AC   ATCC37820;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/vertebrate/E.coli plasmid vector pCYM0 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCYM0 from pSV2-cat & pUT332
RC   pCYM1 from pCYM0
RC   pCYM2 from pCYM0 & YRp7
RC   pCYM3 from pCYM2 & pTG2856
RC   pCYM00 from pCYM0 & pMC1403
RC   pCYM01 from pCYM1 & pMC1403
RC   pCYM02 from pCYM2 & pMC1403
RC   pCYM03 from pCYM3 & pMC1403
RC   pCYM1-11 from pCYM1 & M13mp11
RC   pCYM3-11 from pCYM3 & M13mp11
RA   Camonis J.H., Cassan M., Rousset J.P.;
RT   "Of mice and yeast: versatile vectors which permit gene expression
RT   in both budding yeast and higher eukaryotic cells";
RL   Gene 86:263-268(1990).
RN   [2]
RC   pUT332 from yeast 2 micron & URA3 gene
RA   Sabatier P.;
RT   ;
RL   Unpublished (1990).
CC   The BamHI and EcoRI sites are 3' of the unique available in-phase
CC   initiation codon under control of the SV40 early promoter.
CC   Constructed by inserting the PvuII/BamHI fragment from pSV2-cat (SV40
CC   promoter, small-t intron, polyadenylation signal,inactive CAT) into
CC   pUT322 (2 micron origin, URA3).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.0; BamHI/PvuII--5.6, 0.5; EcoRI--6.0. (ATCC staff)
CC   Shuttle expression vector. (ATCC staff)
CC   Permits expression in both S. cerevisiae and mouse cells. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCYM0)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)
CC   HO (E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse cell line C11D)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. yeast 2 micron 6318bp
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> pUT332 7484bp
FT                   1. pSV2-cat BamHI-PvuII 1486bp 3370..4856,
FT                   \ SV40 prom/small t/polyA/cat
FT                   2. pUT332 large PvuII-BamHI 4900bp,
FT                   \ yeast 2 micron PvuII = 2032 2136, but no BamHI
FT                   -> pCYM0 6360bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;